Dynabeads™ Streptavidin Trial Kit
Dynabeads™ Streptavidin Trial Kit
Invitrogen™

Dynabeads™ Streptavidin Trial Kit

The Dynabeads Streptavidin Trial Kit contains four different streptavidin-coated Dynabeads products making it more convenient to test out which ofRead more
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Catalog number 65801D
Price (USD)
353.00
Each
Add to cart
Price (USD)
353.00
Each
Add to cart

The Dynabeads Streptavidin Trial Kit contains four different streptavidin-coated Dynabeads products making it more convenient to test out which of the beads perform best in a specific application. The kit contains 1 mL each of Dynabeads M-280 Streptavidin, Dynabeads M-270 Streptavidin, Dynabeads MyOne Streptavidin C1, and Dynabeads MyOne Streptavidin T1. The streptavidin-coated Dynabeads magnetic beads are monosized (1 or 2.8 μm in diameter depending on the product), monodispersed, and superparamagnetic. The products are intended for the isolation and handling of biotinylated nucleic acids, antibodies, and other biotinylated ligands.

Benefits of Dynabeads Streptavidin Trial Kit include:
Performance—high sensitivity and reproducibility secure consistent results
Efficiency—fast binding kinetics and high binding capacity
Flexibility—flexible protocols with gentle and efficient liquid-phase reaction kinetics
Automation ready—easy automation for high-throughput needs on the Kingfisher Purification System

Consistency and reproducibility  
The monolayer of streptavidin leaves most of the biotin-binding sites available for the binding of free biotin and biotinylated ligands. The specific and defined surface allows for efficient capture, separation, and downstream handling. In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer help ensure negligible leakage, giving batch consistency and reproducibility of results.

Applications 
Thermo Fisher streptavidin-coupled Dynabeads magnetic beads have been used and cited for a wide variety of applications such as immunoassays, purification of proteins, cells, extracellular vesicles/exosomes, viruses, exosomes, or phage display and bio-panning.

Learn more about our streptavidin products

Automation ready with the KingFisher Purification System 
Dynabeads magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher Sample Purification Systems or liquid handlers.

Commercial supply 
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Streptavidin Trial Kit in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Certifications/ComplianceISO9001 and ISO13485
Concentration10 mg/mL
DescriptionRecombinant streptavidin covalently coupled to Dynabeads
ColorBrown
For Use With (Application)Capture of Biotinylated Targets
MaterialMagnetic Polystyrene
Product TypeStreptavidin Magnetic Beads
FormatBeads in suspension
For Use With (Equipment)KingFisher™ Sample Purification System, DynaMag™ magnets
High-throughput CompatibilityHigh-throughput Compatible
Host SpeciesRecombinant
Isolation TechnologyMagnetic beads
Ligand TypeStreptavidin
PreservativeNaN3
Product LineDynabeads™
Purification TargetBiotin
Quantity4 x 1 mL
Sample TypeAny Sample Type
Shelf Life36 months from date of manufacture
Shipping ConditionAmbient Temperature
Size DistributionCV <5%
Surface FunctionalityTosylactivated-Hydrophobic (M-280 beads and MyOne T1), Carboxylic Acid-Hydrophilic (M-270 beads and MyOne C1)
Volume (Metric)4 x 1 mL
Diameter (Metric)1, 2.8 μm
Regulatory StatusFor Research Use Only
Unit SizeEach
Contents & Storage
The Dynabeads Streptavidin Trial Kit contains 1 mL (10 mg/mL) each of four different streptavidin-coated Dynabeads so you can find the best match for your application.
• Dynabeads M-280 Streptavidin
• Dynabeads M-270 Streptavidin
• Dynabeads MyOne Streptavidin C1
• Dynabeads MyOne Streptavidin T1

Store at 2–8°C.

Frequently asked questions (FAQs)

Can Dynabeads Streptavidin magnetic beads be boiled?

We do not recommend this as streptavidin becomes hydrophobic and aggregates during denaturation.

What is the optimal pH to reduce the settling rate for Dynabeads magnetic beads?

This is dependent on coating or the biotinylated molecule properties. Our recommendation is that this should be tested to find optimum conditions for the specific assay.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

Which methods are common for dissociation of the nonbiotinylated strand of Dynabeads Streptavidin magnetic beads from the biotinylated one?

Several methods can be used.

Using heat:
Wash the DNA-coated Dynabeads magnetic beads in 50µL 1x SSC. (To make SSC, dissolve 0.15 M NaCl, 0.015 M sodium citrate in 800 mL water. Adjust pH to 7.0 with NaOH. Adjust the volume to 1 L with water.)

Resuspend the beads in another 50µL of 1x SSC.

Incubate at 95 degrees C for 5 min.

Quickly put the tube in a magnet stand for 1-2 min and transfer the supernatant to a new tube. The supernatant contains the nonbiotinylated DNA strand.

Generally, heat destabilizes the interaction between biotin and streptavidin and can increase the release of biotinylated ligands from streptavidin. This effect varies in different reagents. In water, normally this effect is minimal, especially if it contains salt.

Using NaOH:< br />
Wash the DNA-coated Dynabeads magnetic beads in 50µL 1 x SSC.

Resuspend the beads in 20 µl of freshly prepared 0.15 M NaOH.

Incubate at room temperature for 10 min.

Put the tube in a magnet stand for 1-2 min and transfer the supernatant to a new tube. The supernatant contains the nonbiotinylated DNA strand.

Neutralize the probe by adding 2.2µL 10 x TE, pH 7.5, and 1.3µL 1.25 M acetic acid. The Dynabeads magnetic beads coated with a biotinylated DNA strand can be washed once with 50µL 0.1 M NaOH, once with 50µL of Binding and Wash buffer, and once with 50µL TE buffer.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

In general, which probe length is better, considering the binding capacity and specificity?

A probe length between 20 and 50 bases is generally okay. The capacity, measured as number of targets captured, is determined by the length of the targets and not as much by the density of probes on the surface.

Which of the Dynabeads Streptavidin magnetic beads should I choose if binding capacity is key?

If binding capacity is of importance, Dynabeads MyOne Streptavidin C1 magnetic beads (Cat. No. 65001) is a good choice.