ViewRNA™ Cell Plus Assay Kit
ViewRNA™ Cell Plus Assay Kit
Invitrogen™

ViewRNA™ Cell Plus Assay Kit

The ViewRNA Cell Plus Assay is a novel assay that combines immunocytochemistry with ViewRNA technology, a proprietary fluorescent in situRead more
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Catalog number 88-19000-99
Price (USD)
2,390.00
Each
Add to cart
Price (USD)
2,390.00
Each
Add to cart
The ViewRNA Cell Plus Assay is a novel assay that combines immunocytochemistry with ViewRNA technology, a proprietary fluorescent in situ hybridization and sequential branched-DNA amplification technique, to visualize both RNA with single-molecule sensitivity and protein in individual cells. This assay enables simultaneous detection of up to three RNA targets in combination with immunophenotyping for cell surface or intracellular proteins using both indirect and direct immunocytochemistry to allow for detailed characterization of specific cell subpopulations. For simultaneous detection of a fourth RNA target, the ViewRNA ISH Cell 740 Module (Cat. No. QVC0200) can be combined with this kit. It allows analysis of an addtional target in the 740 channel (AlexaFluor 750).

This ViewRNA Cell Plus Assay kit contains all the reagents needed to conduct the assay. Target Probe sets for genes of interest and antibodies are sold separately.

Reactivity/species
Probes can be designed for any species. These are the species most frequently used/tested: Armenian hamster, baboon, bovine, canine, cat, chicken, chimpanzee, cow, cynomolgus monkey, donkey, goat, golden Syrian hamster, guinea pig, hamster, horse, human, macaque, monkey, mouse, non-human primate, olive baboon, pig, pigtailed macaque, rabbit, rat, rhesus monkey, sheep

Reported application
Microscopy, immunocytochemistry

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe Detection
FormatKit
For Use With (Application)Microscopy, Immunocytochemistry
Product TypeRNA In Situ Hybridization Assay Kit
Quantity1 kit
Unit SizeEach

Frequently asked questions (FAQs)

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

To perform ViewRNA assays, which incubator oven should I use, Cat. No. QS0704 or QS0712?

Cat. No. QS0704 is a 120 V unit, for use in US/Canada region (https://www.thermofisher.com/order/catalog/product/QS0704).

Cat. No. QS0712 is a 220 V unit, for use in European region (https://www.thermofisher.com/order/catalog/product/QS0712).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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