StemPro™ MSC SFM
StemPro™ MSC SFM
Gibco™

StemPro™ MSC SFM

StemPro™ MSC SFM is a serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (MSCs).
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Catalog NumberWith AdditivesWithout Additives
A1033201Low Glucose, Sodium Pyruvate, Sodium Bicarbonate, Adenosine, DeoxyribonucleosidesNo Glutamine, No Phenol Red
Catalog number A1033201
Price (USD)
483.00
Each
Add to cart
With Additives:
Low Glucose, Sodium Pyruvate, Sodium Bicarbonate, Adenosine, Deoxyribonucleosides
Without Additives:
No Glutamine, No Phenol Red
Price (USD)
483.00
Each
Add to cart
StemPro™ MSC SFM is a serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (MSCs). StemPro™ MSC SFM enables superior human MSC growth and increased consistency compared to classical serum-supplemented medium (DMEM + 10% FBS).

Using StemPro™ MSC SFM, human MSCs can be expanded beyond 5 passages while still maintaining their tri-lineage mesoderm differentiation potential (i.e., ability to differentiate into osteogenic, chondrogenic and adipogenic lineages). Each lot of StemPro™ MSC SFM is qualified using a human MSC performance assay and human MSCs growing in serum-supplemented medium can be transitioned directly into StemPro™ MSC SFM with little or no adaptation required. In addition, StemPro™ MSC SFM facilitates expansion of MSCs directly from primary human bone marrow.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Culture EnvironmentCO2
Cell TypeMesenchymal Stem Cells
ClassificationSerum-free
Culture TypeAdherent Cell Culture
FormLiquid
Product TypeStem Cell Serum Free Media (SFM)
Serum LevelSerum-free
With AdditivesLow Glucose, Sodium Pyruvate, Sodium Bicarbonate, Adenosine, Deoxyribonucleosides
Without AdditivesNo Glutamine, No Phenol Red
Endotoxin LevelLow (≤10 EU/mL)
Inorganic SaltsCalcium, Sodium Phosphate
Product LineStemPro™
Purity or Quality GradeResearch Grade
Quantity1 Kit
SpeciesHuman
Unit SizeEach
Contents & Storage
This kit is shipped in two parts with separate storage requirements. These components are not sold individually:
• StemPro™ MSC SFM Basal Medium, 500 mL (ships at room temperature, store in the dark at 2°C to 8°C)
• StemPro™ MSC SFM Supplement, 75 mL (ships on dry ice, store in the dark at -5°C to -20°C)

Frequently asked questions (FAQs)

Can StemPro MSC SFM (Cat. No. A1003201) be used to isolate primary cells?

Yes. StemPro MSC SFM (Cat. No. A003201) can be used for both isolating primary cells and transitioning cells from serum-containing medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do StemPro MSC SFM Basal Medium CTS and StemPro MSC SFM Supplement CTS contain L-Glutamine?

Both CTS StemPro MSC SFM and StemPro MSC SFM Supplement (Cat. No. A1033201) do not contain L-Glutamine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).