BrightComp eBeads™ Compensation Bead Kits
BrightComp eBeads™ Compensation Bead Kits
Invitrogen™

BrightComp eBeads™ Compensation Bead Kits

BrightComp eBeads compensation beads provide a consistent, accurate, and simple-to-use technique for setting flow cytometry compensation when using fluorescent protein-expressing samples.
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Catalog NumberDescription
A10514GFP BrightComp eBeads™ Compensation Bead Kit
A54740RFP BrightComp eBeads™ Compensation Bead Kit
A54741YFP BrightComp eBeads™ Compensation Bead Kit
A54742CFP BrightComp eBeads™ Compensation Bead Kit
A54743mCherry BrightComp eBeads™ Compensation Bead Kit
Catalog number A10514
Price (USD)
324.00
Each
Add to cart
Description:
GFP BrightComp eBeads™ Compensation Bead Kit
Price (USD)
324.00
Each
Add to cart
BrightComp eBeads compensation beads provide a consistent, accurate, and simple-to-use technique for setting flow cytometry compensation when using fluorescent protein-expressing samples.
• Three levels of intensity, perfect for any samples no matter the level of fluorescent protein expression
• Near-identical, spectrally-matched dye to native protein
• Consistent, reliable performance

Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Each kit provides polystyrene microspheres with near-identical, spectrally-matched fluorescent protein dye as well as negative control beads in a single dropper bottle.
Specifications
ColorGreen
DescriptionGFP BrightComp eBeads™ Compensation Bead Kit
Excitation Wavelength Range488/525
For Use With (Equipment)Flow Cytometer
Shipping ConditionRoom Temperature
For Use With (Application)Flow Cytometry
Product LineeBeads™
Product TypeCompensation Beads
Unit SizeEach
Contents & Storage
Store at 2°C to 8°C. DO NOT FREEZE.

Frequently asked questions (FAQs)

Can I use the GFP BrightComp eBeads to compensate for FITC or Alexa Fluor 488?

No. You must always compensate with the same fluorophore present on the samples. The GFP BrightComp eBeads Compensation Bead Kit spectrally emulates GFP and must only be used on GFP-expressing samples.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I used the GFP BrightComp eBeads Compensation Bead Kit. My peaks are broader than what I see in the manual (Figure 1). What is causing this?

This may be caused by aggregation of the beads. Mix thoroughly before dispensing a drop from the stock vial and mix again after adding buffer. Aggregation may be also be caused by microbial contamination or if the beads were frozen. In these cases, they can no longer be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What material are the beads in the GFP BrightComp eBeads Compensation Bead Kit composed of?

These are polystyrene beads.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.