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RiboMinus™ Eukaryote Kit for RNA-Seq
The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a methodRead more
Catalog number A1083708
Price (USD)
922.00
Each
Price (USD)
922.00
Each
The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.
The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:
• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology
Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module.
The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:
• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology
Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologyMagnetic Bead
Sample TypeTotal RNA
Final Product TypeTranscriptome RNA
High-throughput CompatibilityHigh-throughput Compatible
For Use With (Application)Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
No. of Reactions8 Preps
Product LineRiboMinus™
Quantity1 Each
Sequencing TypeTranscriptome Sequencing
Shipping ConditionWet Ice
Starting Material Amount2-10 μg Total RNA
TargetTranscriptome RNA
System TypeRiboMinus
Yield1 μg
Unit SizeEach
Contents & Storage
Each kit contains:
• 6 mL RiboMinus™ Magnetic Beads
• 80 μL RiboMinus™ Eukaryote Probe (15 pmol/μL)
• 10 mL Hybridization Buffer
• 12 mL DEPC-treated (RNase-free) water
Store at 4°C.