Search Thermo Fisher Scientific
Invitrogen™
ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System
The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cellRead more
Catalog number A11141
Price (USD)
5,340.00
Each
Price (USD)
5,340.00
Each
The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen's ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3/ TO/V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
- Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
- Easy recombination-based cloning using Gateway™ technology
- Stable, long-term, tetracycline-regulated expression
- Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems
Key Features
- WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, increases transgene expression and cPPT (central Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
- Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
- Blasticidin selection marker for stable selection under control of SV40 promoter
Kit includes
- ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit (Cat. No. A11144)
- ViraPower™ Bsd Lentiviral Support Kit (K497000)
- 293FT Cell Line (R70007)
- Gateway™ LR Clonase™ II Plus Enzyme Mix (12538120)
- One Shot™ Stbl3™ Chemically Competent E. coli (C737303)
- Geneticin™ (10131035)
- pENTR™ Gus positive control plasmid
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance BacterialAmpicillin (AmpR), Blasticidin (BsdR)
Cloning MethodGateway
Constitutive or Inducible SystemInducible
Delivery TypeLentiviral
Cell Type293-FT
FormatKit
PromoterCMV/TO
VectorpLenti
Gateway EnzymeLR Clonase™ II Plus, LR Clonase™ II
For Use With (Equipment)Gateway™, HiPerform™, T-REx™, ViraPower™
For Use With (Application)Protein Expression
Inducing AgentDoxycycline, Tetracycline
Key FunctionsViral Expression
No. of Reactions20 Reactions
PCR EnzymeProof-Reading Polymerase
Product LineGateway™, HiPerform™, T-REx™, ViraPower™
Product TypeMammalian Expression system
Protein TagV5 Epitope Tag
Selection Agent (Eukaryotic)Blasticidin
Replication OriginpUC Origin
Selection Marker EukaryoticBsdR
Selection Marker PromoterPGK Promoter
Special ElementsWPRE, cPPT
Unit SizeEach
Contents & Storage
ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit (Cat. No. A11144)
• pLenti6.3/TO/V5-DEST vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg
• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg
• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg
• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C, protect from light.
Store at -20°C unless otherwise noted
ViraPower™ Bsd Lentiviral Support Kit (Cat. No. K497000)
• ViraPower™ Lentiviral Packaging Mix (Contains 195 μL of a mixture of pLP1, pLP2, and pLP/VSVG plasmids, at 1 μg/μL in TE Buffer, pH 8.0), 195 μg. Store at -20°C.
• Lipofectamine™ 2000 (Cat. No. 11668027), 0.75 mL. Store at 4°C (Do not freeze)
• Blasticidin powder (Cat. No. R21001), 50 mg. Store at -20°C.
293FT Cell Line (Cat. No. R70007)
• 3 x 106 frozen cells in 1 mL of Freezing Medium, 1 vial. Store in liquid nitrogen
Gateway™ LR Clonase™ II Plus Enzyme Mix (Cat. No. 12538120)
• Gateway™ LR Clonase™ II Plus Enzyme Mix, 40 μL. Store -20°C for up to 6 months, -80°C for long term storage.
• Proteinase K Solution (2 ug/mL), 40 μL
One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)
• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL. Store at -80°C.
Geneticin™ (Cat. No. 10131035)
• Geneticin™ solution (50 mg/mL), 20 mL, store at 4°C or -20°C
• pENTR™ Gus positive control plasmid, store at -20°C
pUC19 control DNA, (10 pg/μL), 50 μL