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TrypLE™ Select Enzyme (10X), no phenol red
TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO,Read more
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| Catalog Number | Quantity |
|---|---|
| A1217703 | 20 x 100mL |
| A1217701 | 100 mL |
| A1217702 | 500 mL |
Catalog number A1217703
Price (USD)
2,785.00
Each
Quantity:
20 x 100mL
Price (USD)
2,785.00
Each
TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.
Compared to trypsin and other dissociation reagents, Gibco™ TrypLE™ Select is:
• Gentle on cells—maintains cell health for reproducible results
• Room-temperature stable—convenient to use
• Easy to use—substitutes directly into existing protocols, with no inactivation required
• Animal origin free (AOF)—pure, consistent, and effective
This TrypLE™ Select is modified as follows:
With
• EDTA
Without
• Phenol Red
The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.
Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.
Product Intended Use
For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco™ TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
Gibco™ TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.
Compared to trypsin and other dissociation reagents, Gibco™ TrypLE™ Select is:
• Gentle on cells—maintains cell health for reproducible results
• Room-temperature stable—convenient to use
• Easy to use—substitutes directly into existing protocols, with no inactivation required
• Animal origin free (AOF)—pure, consistent, and effective
This TrypLE™ Select is modified as follows:
With
• EDTA
Without
• Phenol Red
The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.
Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.
Product Intended Use
For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco™ TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
Gibco™ TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
Specifications
ChelatorsEDTA
ClassificationAnimal Origin-free
Concentration10 X
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeCell Culture Dissociation Reagent
Shelf Life24 Months
Without AdditivesNo Phenol Red
Green FeaturesEnergy efficient, Sustainable packaging
Inorganic SaltsNo Calcium, No Magnesium, No Sodium Phosphate
Osmolality270 - 320 mOsm/kg
pH6.9 to 7.4
Product LineGibco™
Purity or Quality GradeCell Culture Grade
Quantity20 x 100mL
RemarksRoom Temperature Stable
Shipping ConditionRoom Temperature
Tested ForIn vitro Bioassay
Unit SizeEach
Contents & Storage
Storage conditions: 15–30°C. Protect from light.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.