CTS™ TrypLE™ Select Enzyme

Gibco™

CTS™ TrypLE™ Select Enzyme

Name: CTS™ TrypLE™ Select Enzyme
SKU: A1285901
Price: 68.75 USD

TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. The GIBCO™ CTS™Read more

Catalog number A1285901

Price (USD)

68.75

Each

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Price (USD)

68.75

Each

Add to cart

TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. The GIBCO™ CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.

• GIBCO™ TrypLE™ Select ™ CTS™ enables you to reduce your burden in qualifying reagents during your transition from research to clinical applications.
• Non-animal or human origin
• Reduced risk of viral or prion contamination
• Removal kinetics and recovery viability comparable to porcine trypsin
• Demonstration of activity on multiple cell lines, including strongly attached lines
• Stable at room temperature (15° to 30°C) for up to 24 months.
• Manufactured using dedicated, autoclaved or disposable equipment.

TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. This material is used for the dissociation of attachment-dependent cell lines from plasticware. TrypLE Select has demonstrated the ability to dissociate cells cultured both in serum-free and serum supplemented systems. The product is provided as a 1X stock solution, formulated in DPBS with 1mM EDTA. The concentration of rProtease is proprietary to
Invitrogen Corp.

GIBCO™ CTS™ products are of high quality, and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate. The GIBCO™ CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.

Specifications

Cell TypeMammalian Cells

ClassificationAnimal Origin-free

Concentration1X

FormLiquid

Product TypeCell Culture Dissociation Reagent

Shelf Life24 months from date of manufacture

SterilitySterile-filtered

Without AdditivesNo Phenol Red

FormatBottle(s)

For Use With (Application)Cell and Gene Therapy Research, Development, and Manufacturing

Green FeaturesSustainable packaging

Manufacturing QualityISO 13485, MDSAP, FDA-registered, 21 CFR 820

Quantity100 mL

Shipping ConditionRoom Temperature

Unit SizeEach

Contents & Storage

Store at room temperature.

Frequently asked questions (FAQs)

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there an upper limit for the amount of time cells can be incubated with TrypLE Express Enzyme, TrypLE Select Enzyme, or CTS TrypLE Select Enzyme for dissociation?

There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For your TrypLE products, how does the enzyme get inactivated?

Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:

After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.