CTS™ KnockOut™ DMEM/F-12
CTS™ KnockOut™ DMEM/F-12
Gibco™

CTS™ KnockOut™ DMEM/F-12

CTS™ KnockOut™ DMEM/F-12 CTS™ is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized forRead more
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Catalog number A1370801
Price (USD)
68.25
Each
Add to cart
Price (USD)
68.25
Each
Add to cart

CTS™ KnockOut™ DMEM/F-12 CTS™ is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). KnockOut™ DMEM/F-12 CTS™ media is designed for use with KnockOut™ SR and KnockOut™ SR XenoFree CTS™ supplements.

• Designed for reliable and consistent performance
• Perform ESC/iPSC culturing with little to no spontaneous differentiation
• Be ready for clinical research with this cGmP manufactured formula and support documentation

Consistent Results Without Fetal Bovine Serum
Cell Therapy Systems KnockOut™ DMEM/F-12 CTS™ media is part of the KnockOut™ line of media products that have delivered reliable results for ESC and now iPSC culturing for years. This fetal bovine free media leads to little or no spontaneous differentiation, and frees you from repeated screens.

Equivalent Morphology
Stem cells cultured in KnockOut™ DMEM/F-12 CTS™ media show normal morphology and karyotype when compared to control stem cells. You can also expect equivalent morphology to manually passaged hESCs, even after enzymatic passaging.

Simplify Your Transition into Clinical Research
The Gibco™ Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco™ Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.
Specifications
Cell TypeEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
ClassificationAnimal Origin-free
FormLiquid
Product TypeCell Culture Medium
Shelf Life18 months from date of manufacture
SterilitySterile-filtered
FormatBottle
For Use With (Application)Cell and Gene Therapy Research, Development, and Manufacturing
Green FeaturesSustainable packaging
Manufacturing QualityISO 13485, MDSAP, FDA-registered, 21 CFR 820
Quantity500 mL
Shipping ConditionAmbient
Unit SizeEach
Contents & Storage
Store at 2–8°C and protect from light.

Frequently asked questions (FAQs)

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all CTS™ KnockOut™ DMEM/F-12 FAQs