Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix
Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix
Gibco™

Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix

Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-SwarmRead more
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Catalog NumberQuantity
A14133011 mL
A14133025 mL
Catalog number A1413301
Price (USD)
84.25
Each
Add to cart
Quantity:
1 mL
Price (USD)
84.25
Each
Add to cart
Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. Routinely used for attachment and maintenance of human embryonic stem cells (hESC), every lot of Geltrex™ LDEV-free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is quality control tested using hESCs and StemPro hESC Serum Free Media to help ensure growth and pluripotency of each lot.

Key Features of Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix:

• Every lot is qualified using hESC, which helps reduce the need for customers to lot screen
• Free of the lactose dehydrogenase elevating virus (LDEV) making it safe for all types of cell culture
• Consistent protein concentration lot-to-lot helps ensure reproducible results

Gain Reliability and Consistency in Your hESC Studies
Human ESCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support. Geltrex™ hESC-qualified reduced growth factor basement membrane matrix has been tested for support of hESC growth and pluripotency, therefore minimizing the need for you to test multiple lots to find one that works. Our consistent manufacturing process helps ensure a protein concentration averaging 15 mg/ml with very little variation from lot-to-lot, so you can have confidence in every purchase.

Free of LDEV Contamination
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus-free.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeHuman induced pluripotent stem cells (iPSC), primary epithelial cells, endothelial cells, smooth muscle cells
ClassificationReduced Growth Factor
Concentration12 to 18 mg/mL
Culture TypeAdherent Cell Culture
FormFrozen
Product TypeBasement Membrane Matrix
Serum LevelSerum-free
Shelf Life36 months
SterilitySterile
Without AdditivesNo Phenol Red
Endotoxin LevelLow
FormatTube(s)
Product LineGibco™
Purity or Quality GradeCell Culture Grade
Quantity1 mL
Shipping ConditionDry Ice
SpeciesMouse
Unit SizeEach
Contents & Storage
Geltrex™ LDEV-Free hESC-qualified comes as a sterile protein solution in 1 mL vials.

Store at -20° to -80°C.

Geltrex™ Matrix is stable for 18 months from date of manufacture when properly stored.

Frequently asked questions (FAQs)

Can I use the Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601) with other PSC media systems (i.e. StemFlex, Essential 8, Essential 8 Flex, etc.)?

No, the Ready-To-Use Geltrex matrix was developed for use with the StemPro hESC SFM System. It is not recommended for other PSC media systems.

For other PSC media systems, we recommend the Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Geltrex LDEV-Free and Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement?

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601) is a pre-diluted Geltrex product ready to use for coating culture vessels. There is no need to dilute the solution further. The product has also been QC tested using hESCs to ensure that every lot will maintain the attached pluripotency of these cells. The full product description can be found here:

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601)

Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302) is a "concentrated" version of the Geltrex hESC-Qualified, Ready-To-Use above. You can pre-dilute this concentrated solution to pre-coat the culture vessel to grow hESCs at your desired usage concentration. This Geltrex matrix has also been QC tested using hESCs to ensure every lot will maintain the attached pluripotency of these cells. The full product description can be found here:

Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302)

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202) is the same as the product above. However, it has not been tested to verify it will support hESC growth and expansion. This is a "general" version and not specific to a cell type or line. A full product description can be found here:

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202)

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What volume do you recommend for thin coating 35 mm dishes with Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413201, A1413202) or Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302)?

We recommend thin (non-gelling) coating 35 mm dishes with 1.5 mL of Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413201, A1413202) or Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302) prediluted 1/100 in DMEM/F-12 medium (1% final concentration).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I prepare Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302) stock solution, and how long is the stock solution stable after thawing?

We recommend thawing Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. 1413301, 1413302) at 4 degrees C before making your stock solution. On ice, make a 1:1 dilution with DMEM/F-12 and aliquot into smaller volumes. Stock solution aliquots should be stored at -20 degrees C or used immediately. Once the stock solution is thawed, it should be used for coating plates immediately.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix and Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix? I am working with human hepatocytes.

We do not have comparison data for the two Geltrex products. We recommend using the Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix for hepatocytes. We have not performed a significant amount of testing using the Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix on these cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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