ExpiFectamine™ 293 Transfection Kit
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ExpiFectamine™ 293 Transfection Kit
Gibco™

ExpiFectamine™ 293 Transfection Kit

The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfectionRead more
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Catalog NumberQuantity
A14524For 1 L of culture
A14525For 10 L of culture
A14526For 50 L of culture
Catalog number A14524
Price (USD)
964.00
Each
Add to cart
Quantity:
For 1 L of culture
Price (USD)
964.00
Each
Add to cart

The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium

  • Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
  • Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
  • Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
  • Provides robust and reproducible transfection results, giving users greater confidence in their results
  • Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields

The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2. The enhancers are added 18 to 22 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin™ and FreeStyle™ MAX.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineExpi293F™
ClassificationAnimal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Cell TypeEstablished Cell Lines
Sample TypePlasmid DNA
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Product LineExpiFectamine™
Product TypeTransfection Kit
PurityCell Culture Grade
QuantityFor 1 L of culture
Serum CompatibleNo
Unit SizeEach
Contents & Storage
2.7 mL (3 x 0.9 mL) ExpiFectamine™ 293 Reagent
5 mL ExpiFectamine™ 293 Transfection Enhancer 1
50 mL ExpiFectamine™ 293 Transfection Enhancer 2

Sufficient for transfection of 1 L of culture

Store at 2°C to 8°C

Frequently asked questions (FAQs)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

Is it necessary to use serum-free medium during lipid transfection?

It is not necessary to use serum-free medium during lipid transfection. However, it is critical to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum-containing medium. For optimal results with Lipofectin Transfection Reagent, we recommend performing transfection in medium without serum.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

View all ExpiFectamine™ 293 Transfection Kit FAQs