Vitronectin (VTN-N) Recombinant Human Protein, Truncated
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Vitronectin (VTN-N) Recombinant Human Protein, Truncated

Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cellsRead more
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Catalog NumberVolume (Metric)
A147001 mL
A3180410 mL
Catalog number A14700
Price (USD)
87.50
Each
Add to cart
Volume (Metric):
1 mL
Price (USD)
87.50
Each
Add to cart
Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium (A1517001), vitronectin (VTN-N) has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines. In addition, vitronectin (VTN-N) has been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. As published by Guokai Chen et al. (1) in the laboratory of James Thomson, the VTN-N variant of vitronectin supports human pluripotent stem cell attachment and survival better than wild-type vitronectin when used in conjunction with Essential 8™ Medium.

Vitronectin (VTN-N) is:

Optimized—designed in the laboratory of James Thomson for use with Essential 8™ Medium
Defined—for reduced variability in your PSC cultures
Cost effective—for economical and scalable PSC culture

Optimized and Defined Surface
Unlike standard basement membrane extracts (BMEs), vitronectin (VTN-N) was designed in the laboratory of James Thomson for use with Essential 8™ Medium and has been shown to support human pluripotent stem cell attachment and survival better than wild type vitronectin. Vitronectin (VTN-N) provides an optimal environment for feeder-free pluripotent stem cell culture.

Reduce Variability
Vitronectin (VTN-N) is a defined, recombinant human protein that reduces variability in your PSC cultures compared to human-plasma derived vitronectin and standard basement membrane extracts (BMEs).

Cost Effective
When used with Essential 8™ Medium, vitronectin (VTN-N) provides a cost effective, defined system for feeder free culture of human pluripotent stem cells (PSCs).

Commercialized in Partnership with Cellular Dynamics International.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration500 μg⁄ml
For Use With (Application)Stem Cell Culture
Purification MethodHPLC
Quantity1 mL
SpeciesHuman
RecombinantRecombinant
ClassificationAOF
Endotoxin LevelVery Low
Shipping ConditionDry ice
Volume (Metric)1 mL
Protein FormRecombinant
FormLiquid
Protein SubtypeVitronectin
Research CategoryStem Cell Research
Purity or Quality Grade95 %
Unit SizeEach
Contents & Storage
Store at -80°C and protect from light.

Frequently asked questions (FAQs)

What substrate should I use with the PSC Definitive Endoderm Induction Kit?

We recommend using Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A14700), but Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301 or Cat. No. A1413302) can also be used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why are the recommended working concentrations different for CTS Vitronectin (VTN-N) Recombinant Human Protein, Truncated vs. Vitronectin (VTN-N) Recombinant Human Protein, Truncated? Are they not the same protein?

These two proteins are the same but have different protein concentrations due to the different methods used to measure the protein concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for blood cells?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of CD34+ blood cells. For CD34+ cells, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 39-44). On Day 3, you can utilize rhVTN-N (Cat. No. A14700), Geltrex (Cat. No. A1413302), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, reprogrammed CD34+ cells should be fed every-other-day with StemFlex Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for human fibroblasts?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of both neonatal and adult human dermal fibroblasts. For fibroblasts, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 16-20). On Day 7, you may use rhVTN-N (Cat. No. A14700), Geltrex matrix (Cat. No. A1413302), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, we recommend that you feed reprogrammed fibroblasts every-other-day with StemFlex Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

You recommend Geltrex as the matrix for routine culture of StemFlex Medium. Can I also use Vitronectin or rhLaminin-521?

Yes. We recommend following the coating instructions for Vitronectin or rhLaminin-521 and using the culture and passaging recommendations in the StemFlex protocol.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.