B-27™ Supplement, minus insulin
B-27™ Supplement, minus insulin
Gibco™

B-27™ Supplement, minus insulin

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Insulin is a customized version of theRead more
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Catalog NumberQuantity
A189560110 mL
A1895602100 mL
Catalog number A1895601
Price (USD)
175.00
Each
Add to cart
Quantity:
10 mL
Price (USD)
175.00
Each
Add to cart
A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Insulin is a customized version of the original B-27 Supplement without insulin. B-27 Supplement Minus Insulin is a serum-free supplement designed to support growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference. B-27 Supplement Minus Insulin is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Cells
ClassificationSerum-free
Concentration50 X
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeCell Culture Supplement
Serum LevelSerum-Free
Shelf Life12 Months
SterilitySterile
Product LineB-27™
Quantity10 mL
Shipping ConditionDry Ice
SourceSerum-Free
Tested ForEndotoxin, Performance, Sterility, Mycoplasma
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -20°C). Protect from light.

Frequently asked questions (FAQs)

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I thaw B-27 Supplements?

We recommend thawing it at 4 degrees C overnight or at room temperature for 2 hours. We don't recommend thawing in a water bath or incubator.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I re-freeze B-27 Supplements into smaller aliquots for future use?

Yes, we recommend that you aliquot when you first thaw it and thereafter subject the aliquot to no more than 2 additional freeze/thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.