Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit
Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit
Invitrogen™

Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit

The Amplex Red Xanthine/Xanthine Oxidase Assay Kit provides a sensitive and simple fluorometric method for detecting as little as 200Read more
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Catalog number A22182
Price (USD)
451.00
Each
Add to cart
Price (USD)
451.00
Each
Add to cart
The Amplex Red Xanthine/Xanthine Oxidase Assay Kit provides a sensitive and simple fluorometric method for detecting as little as 200 nM xanthine or hypoxanthine or as little as 0.1 mU/mL of xanthine oxidase activity in a purified system with a 100 μL assay volume.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Application)Xanthine/Xanthine Oxidase Assay
For Use With (Equipment)Microplate Reader, Spectrophotometer, Fluorometer
Product TypeRed Xanthine/Xanthine Oxidase Assay Kit
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), 96-well plate
Product LineAmplex™
Quantity400 assays
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.