Alexa Fluor™ 532 Phalloidin
Alexa Fluor™ 532 Phalloidin
Invitrogen™

Alexa Fluor™ 532 Phalloidin

Alexa Fluor 532 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, yellow fluorescent Alexa Fluor 532 dye.•Read more
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Catalog number A22282
Price (USD)
592.00
Each
Add to cart
Price (USD)
592.00
Each
Add to cart
Alexa Fluor 532 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, yellow fluorescent Alexa Fluor 532 dye.

• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/Emission: 531/554 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor 532 provides yellow fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor532 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor 532 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor 532 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
• Read about labeling F-Actin with phallotoxins.
• See the full line of Alexa Fluor Dye products.
• Create a Cellular Masterpiece with Alexa Fluor Phalloidin using the Cell Paint Tool.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation Wavelength Range531⁄554
Dye TypeAlexa Fluor™ 532
Quantity300 units
Product LineAlexa Fluor™
Shipping ConditionRoom Temperature
Label TypeAlexa Fluor Dyes
Product TypePhalloidin
SubCellular LocalizationActin, Cytoskeleton
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

Can I combine Click-iT or Click-iT Plus reactions with phalloidin conjugates used for actin staining?

We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.

For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.

Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?

When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.