MagMAX™ DNA Multi-Sample Ultra Kit (with extra proteinase K and DNA binding beads)
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MagMAX™ DNA Multi-Sample Ultra Kit (with extra proteinase K and DNA binding beads)
Applied Biosystems™

MagMAX™ DNA Multi-Sample Ultra Kit (with extra proteinase K and DNA binding beads)

Note: This product is identical to the MagMAX™ DNA Multi-Sample Ultra Kit (Cat. No. A25597) except that it comes withRead more
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Catalog NumberIncludes
A25920
A25919
Catalog number A25920
Price (USD)
15,280.00
Each
Add to cart
Price (USD)
15,280.00
Each
Add to cart

Note: This product is identical to the MagMAX™ DNA Multi-Sample Ultra Kit (Cat. No. A25597) except that it comes with additional proteinase K and DNA Binding Beads, which some customers find useful for their specific applications.

The MagMAX™ DNA Multi-Sample Ultra Kit is optimized to isolate genomic DNA (gDNA) from a variety of samples, such as whole blood, buccal cells, saliva, urine, blood cards, mouth rinse, and tissue. gDNA purified with the kit is ideal for qPCR applications on sensitive, scalable platforms such as the QuantStudio™ 12K Flex System with OpenArray™ Block (with AccuFill™ System). The magnetic bead–based purification format allows you to easily scale from processing 12 to 500 samples a day, making it the ideal choice for pharmacogenomics (PGx) studies.

Features of the MagMAX™ DNA Multi-Sample Ultra Kit include:
• Streamlined protocols for numerous non-invasive biological samples
• Optimized to provide DNA yields suitable for OpenArray™ and Ion AmpliSeq™ applications
• Packaged for high-throughput needs

One Kit for a Diverse Set of Sample Types
Why purchase multiple purification kits to isolate gDNA from different sample types? The MagMAX™ DNA Multi-Sample Ultra Kit eliminates the headache of ordering these extra kits because it has been optimized for use with a variety of biological samples. The kit is designed for high-throughput isolation of gDNA from 1 buccal swab, 50 to 350 μL of whole blood, up to 400 μL urine (without sample concentration), up to 150 μL saliva, 1-5 mL mouth rinse, blood spot cards, and mammalian tissue samples (up to 10 mg). The protocols and magnetic bead format make the kit fully amenable to automation.

*Please note that the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor with a 24 Deep-well Head is recommended for sample volumes greater than 50 μL.

Benefits of MagMAX™ Magnetic Bead–based Purification
Magnetic beads offer many benefits compared to other technologies for isolating gDNA. Beads bind gDNA more efficiently than glass fiber filters, resulting in higher and more consistent gDNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.

Accessory Products
Proteinase K (Cat. No. A25561), DNA Binding Beads (Cat. No. A25562), Wash Solution 1 Concentrate (Cat. No. AM8504), and Wash Solution 2 Concentrate (Cat. No. AM8640) are components of the kit that may also be purchased separately.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1 ng/μL to 3 ng/μL
FormatKit
Isolation TechnologyMagnetic Bead
Sample TypeBlood, Buccal Samples, Cells, Liquid Samples (e.g. Serum), Tissue
For Use With (Application)Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)
High-throughput CompatibilityHigh-throughput Compatible, Manual Protocols, Automated Protocols
No. of Reactions2500 Preps
Product LineMagMAX™
Product TypeDNA Multi-Sample Ultra Kit
Quantity2500 Preps
TargetGenomic DNA
Unit SizeEach
Contents & Storage
• DNA Binding Beads, 10 x 8 mL (store at 2°C to 8°C, DO NOT FREEZE)
• DNA Elution Buffer 1, 5 x 25 mL (store at room temperature)
• DNA Elution Buffer 2, 5 x 25 mL (store at room temperature)
• Wash Solution 1 Concentrate, 5 x 80 mL* (store at room temperature)
• Wash Solution 2 Concentrate, 5 x 162 mL* (store at room temperature)
• Multi-Sample DNA Lysis Buffer, 5 x 100 mL (store at room temperature)
• Nuclease-Free Water, 5 x 100 mL (store at room temperature)
• PK Buffer, 5 x 96 mL (store at room temperature)
• Proteinase K, 10 x 4 mL (store at -5° to -30°C)
• Ribonuclease A (RNase A), 5 x 1.25 mL (store at -5° to -30°C)
*after addition of alcohol

Frequently asked questions (FAQs)

When using the PowerUp SYBR Green Master Mix (Cat. No. A25741, A25742, A25743, A25776, A25777, A25778, A25779, A25780, A25919), I get high Ct values. What can I do?

It could be due to the following reasons:

  • Insufficient cDNA template is present. Use 10 to 100 ng of cDNA template per 50 µL reaction.
  • Quality of cDNA template is poor. Quantify the amount of cDNA template and test the cDNA template for the presence of PCR inhibitors.
  • Sample degradation. Prepare fresh cDNA, then repeat the experiment.
  • Reduced number of PCR cycles in the thermal cycling protocol. Increase the number of PCR cycles to the default setting of 40.
  • Primer-dimer formation and residual polymerase activity. Prepare the reaction mixes and reaction plate on ice. To ensure optimal results, run the reaction plate as soon as possible after completing the reaction setup.


Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

When using the PowerUp SYBR Green Master Mix (Cat. No. A25741, A25742, A25743, A25776, A25777, A25778, A25779, A25780, A25919), the Multicomponent signal for ROX is not linear. What could cause this?

This could be due to the following reasons:

  • Pure dye component’s spectra are incorrect. Rerun pure dye spectra.
  • Incorrect dye components have been chosen. Choose correct dyes for data analysis.


Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Which reverse transcription kit can I use with the PowerUp SYBR Green Master Mix (Cat. No. A25741, A25742, A25743, A25776, A25777, A25778, A25779, A25780, A25919)?

We recommend using the High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406, 4388950).

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What primer concentrations can I use with the PowerUp SYBR Green Master Mix (Cat. No. A25741, A25742, A25743, A25776, A25777, A25778, A25779, A25780, A25919)?

The recommended primer concentration range is 300-800 nM. You will need to optimize the primer concentration by running different concentration combinations of the forward and reverse primer. See page 28 of the PowerUp SYBR Green Master Mix User Guide.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What type of samples is the MagMAX DNA Multi-Sample Ultra Kit (Cat. No. A25597) optimized for?

We have optimized protocols for extracting DNA from buccal swabs, whole blood, blood cards (Whatman FTA), oral rinse, saliva, and urine samples. In addition, the kit can be used to process vaginal microbiota samples (e.g., ThinPrep, Surepath) for gDNA extraction from yeast, bacteria (Gram positive/negative), viruses, and protozoa with the addition of additional enzymatic steps.