TaqPath™ 1-Step Multiplex Master Mix
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TaqPath™ 1-Step Multiplex Master Mix
Applied Biosystems™

TaqPath™ 1-Step Multiplex Master Mix

TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of upRead more
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Catalog NumberQuantity
A285265 x 1 mL
A285250.5 mL
A2852710 mL
Catalog number A28526
Price (USD)
2,695.00
Each
Add to cart
Quantity:
5 x 1 mL
Price (USD)
2,695.00
Each
Add to cart

TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction. Particularly useful for diagnostics and biopharmaceuticals with applications in viral pathogen detection and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, MUSTANG PURPLE dye (passive reference), and thermostable Fast DNA polymerase all in a single tube. TaqPath is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

This single-tube, 4X format facilitates easy reaction set up—just add user-supplied assay and sample (Figure 1). With reproducible performance even in the presence of inhibitors, coupled with stringent quality control to minimize lot-to-lot variation, TaqPath 1-Step Multiplex Master Mix is a superior choice for your multiplex diagnostic or bioprocess quality control testing and development needs.

Features of the TaqPath 1-Step Multiplex Master Mix include:
High sensitivity—to detect low-copy targets with reproducible CT results
Broad dynamic range—with both RNA and DNA targets
Optimized for multiplexing—up to four targets simultaneously with MUSTANG PURPLE reference dye
Tolerant of inhibitors—commonly found in clinical and biopharma samples
Advanced manufacturing—production and process controls ensure lot-to-lot consistency
Compliance Package—containing most frequently requested compliance documents, available below, commonly used to comply with internal quality systems and regulatory requirements in molecular diagnostic assay and drug development

High sensitivity
To enable reproducible detection of low-titer pathogen and transcripts, TaqPath 1-Step Multiplex Master Mix has been optimized as a higher-concentration 4X master mix that allows you to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Figure 1 shows the consistency of results obtained when detecting 10 copy inputs of RNA target.

Wide dynamic range compatible with RNA and DNA
TaqPath 1-Step Multiplex Master Mix has been optimized to provide high specificity and dynamic range for use with both RNA and DNA targets. Since virology labs often test for both RNA and DNA viruses, TaqPath 1-Step Multiplex Master Mix is designed to use a single protocol to assay both types of nucleic acid. This input flexibility can help streamline the number of different workflows in your lab to improve efficiency. Figure 2 demonstrates the excellent PCR linearity across a 6-log RNA dilution series, while Figure 3 exhibits a 6-log linear dynamic range for both RNA and DNA targets.

Optimized for multiplexing
TaqPath 1-Step Multiplex Master Mix has been validated for multiplexing up to four targets simultaneously, allowing for additional targets and/or controls to be run simultaneously for efficiency or quality control purposes. Rather than including ROX dye as a passive reference, the TaqPath 1-step Multiplex Master Mix includes the longer emission wavelength MUSTANG PURPLE dye as a passive reference. This longer wavelength passive reference dye enables measurement of the JUN dye, or a similar emission wavelength dye, in the channel previously used to measure ROX dye. Figure 3 demonstrates TaqPath 1-Step Multiplex Master Mix performance in 4-plex reactions with both RNA and cDNA targets.

Inhibitor tolerant
Unlike other master mixes on the market, the unique proprietary formulation of TaqPath 1-Step RT-qPCR Master Mix allows robust performance even in the presence of PCR inhibitors typically found in biopharmaceutical, molecular diagnostics, and research applications. Optimized performance in the presence of such substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, helps increase confidence when working with a variety of complex clinical or biopharma samples. TaqPath master mixes demonstrate increased tolerance of inhibitors compared to competitor mixes (Figure 4).

Manufacturing production and process controls
TaqPath 1-Step Multiplex Master Mix is manufactured under a strong quality management system that utilizes traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath product manufacturing is designed to produce consistent product performance lot after lot. The stringent QC is important for a variety of applications, making the master mix ideal for bioprocess quality control testing, biologics adventitious testing, molecular diagnostics, and research applications.

General Purpose Reagent manufactured under cGMP
TaqPath master mixes are general purpose reagents labeled “For Laboratory Use” and manufactured in an ISO 13485-certified and FDA-registered facility that adheres to cGMP principles.

Compliance Package
In an effort to efficiently support your compliance needs, frequently requested compliance documents are available here:
Statement of Quality relating to the current good manufacturing practices (cGMP) to which TaqPath master mixes adhere
• Detailed lot-specific Certificate of Analysis (COA) (see below in Documents & Downloads section)
Product Stability Statement
• Supplier Evaluation Support
   -- Completed Self Assessment
   -- ISO certificates by manufacturing site
• For EU Reach compliance-related inquiries, email 'LSGChemicalCompliance@thermofisher.com'
• BSE/TSE Statement
• Change Control and Notification Registration
Not finding the compliance document you’re looking for? Technical Support is here to help.

Specifications
Detection MethodPrimer-probe
FormatTube
PCR Method1-step RT-qPCR, Multiplex qPCR
PolymeraseFast DNA Polymerase
Reaction SpeedFast or Standard
For Use With (Equipment)QuantStudio™ 6 Pro Real-Time PCR System or QuantStudio™ 7 Pro, QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, ViiA™ 7, 7500 , 7500 Fast, 7500 Fast Dx
Product LineTaqPath™
Product TypeqPCR Master Mix
Quantity5 x 1 mL
Shipping ConditionDry Ice
Sufficient For1000 Reactions of 20 μL
For Use With (Application)Gene Expression
Concentration4X
No. of Reactions1000 Reactions
Sample TypeDNA, RNA
Volume5 x 1 mL
Unit SizeEach
Contents & Storage
Contains 5 x 1 mL tubes of 4 x RT-qPCR master mix sufficient for 1000 x 20 μL reactions.

Store at -10°C to -30°C in the dark.

Frequently asked questions (FAQs)

I need to multiplex but cannot use ROX. What options do I have?

For multiplex assays that include reporter dyes overlapping the ROX channel (i.e. JUN), we recommend using one of our optimized multiplex master mixes that include the passive reference dye, MUSTANG PURPLE. MUSTANG PURPLE is compatible with QuantStudio 5, 6, 7, and 12K Flex, 7500, 7500 Fast, and ViiA 7 real-time PCR instruments.

While not ideal, we also offer master mixes that contain no passive reference dyes. For selection help for master mixes with no passive reference, please contact Technical Support at techsupport@thermofisher.com.

What master mix should I use for multiplexing in my qPCR experiments?

The choice of master mix depends upon the number of assays in your reaction, the application, and instrument compatibility. Please refer to our real-time PCR master mixes and instrument compatibility chart (https://assets.thermofisher.com/TFS-Assets/LSG/brochures/master-mix-instrument-compatibility.pdf) prior to selecting one of the master mixes below:

Gene Expression Assays:
- Single, duplex, and triplex assays: TaqMan Fast Advanced Master Mix or TaqMan Fast Virus 1-Step Master Mix
- Multiplexing up to 4 assays: TaqMan Multiplex Master Mix or TaqPath 1-Step Multiplex Master Mix

SNP Genotyping Assays:
- Single SNP Genotyping assay: TaqPath ProAmp Master Mix
- Duplex SNP Genotyping assays: TaqPath ProAmp Multiplex Master Mix, TaqMan Multiplex Master Mix

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.