Ion AmpliSeq™ Kit for Chef DL8
Get NGS panels in smaller pack sizes. Simply select genes from a catalog. Learn more »
Ion AmpliSeq™ Kit for Chef DL8
Ion Torrent™

Ion AmpliSeq™ Kit for Chef DL8

The Ion AmpliSeq™ Kit for Chef DL8 is designed for use with the Ion Chef™ System for automation of IonRead more
Have Questions?
Catalog number A29024
Price (USD)
6,040.00
Each
Add to cart
Price (USD)
6,040.00
Each
Add to cart

The Ion AmpliSeq™ Kit for Chef DL8 is designed for use with the Ion Chef™ System for automation of Ion AmpliSeq library construction.

This kit offers:

  • Simplest workflow for targeted sequencing library preparation using 1 or 2 pool Ion AmpliSeq panel designs*
  • Low input
  • Easy-to-use cartridge format with reagent barcoding for full reagent tracking
  • Proven performance of Ion AmpliSeq technology, compatible with DNA extracted from formalin-fixed, paraffin-embedded tissue and other challenging sources

*See AmpliSeq.com for custom 1-2 pool panels. Note that some panels (e.g., Transcriptome (Cat. No. A31446), Cancer Hotspot (A32914), Oncomine BRCA (A32841), Pharmacogenomics (A29998)) have been prepared in an Ion Chef-ready kit by incorporating the respective ready-made primer pool with the DL8 kit.

Leveraging the automation capabilities of the Ion Chef System, the Ion AmpliSeq Kit for Chef DL8 enables the reproducible preparation of Ion AmpliSeq amplicon libraries, with less than 15 minutes of hands-on time and only 3 liquid transfer steps. Compatible with 1- and 2-pool Ion AmpliSeq panel designs, the kit automates the preparation of libraries from 8 samples in a single run, delivering a single tube of pooled libraries ready for downstream templating and sequencing. The kit provides sufficient material for the preparation of 32 libraries (4 Ion Chef runs).

Utilizing plug and play, pre-packaged, single-use cartridges and PCR plates for a fully automated workflow, users of all experience levels will find library preparation straightforward with minimal effort. Supplied with barcodes conveniently dried-down in 96-well plates, multiplexing samples is easy and requires no additional pipetting steps.

Specifications
FormatKit
Sample TypeDNA
Sequencing TypeGenome and DNA Sequencing
For Use With (Equipment)Ion GeneStudio™ S5 Series Sequencer, Ion Chef™ System
For Use With (Application)Sequencing
LibrariesTargeted Sequencing Library
Product LineAmpliSeq™
Product TypeDNA Sequencing Kit
Quantity32 reactions
Shipping ConditionDry Ice
Workflow StepLibrary Generation
Unit SizeEach
Contents & Storage
• Ion AmpliSeq Chef Reagents DL8, 4 cartridges, store at -5°C to -30°C
• Ion AmpliSeq Chef Solutions DL8, 4 cartridges, 2°C to 8°C
• Ion AmpliSeq Supplies (Each box contains: Ion AmpliSeq Tip Cartridge L8, Framed PCR Foil Seal, Enrichment Cartridge), 4 boxes, room temperature
• IonCode in PCR Plates (The set includes: IonCode 0101-0108 in 96-well PCR Plate (red), IonCode 0109-0116 in 96-well PCR Plate (yellow), IonCode 0117-0124 in 96-well PCR Plate (green), IonCode 0125-0132 in 96-well PCR Plate (blue)), 1 set of 4, room temperature

Frequently asked questions (FAQs)

What is the difference between a DNA fragment library and a DNA amplicon library?

A DNA fragment library is constructed from whole genomic DNA and is commonly used for whole genome resequencing or de novo sequencing. Briefly, the whole genomic DNA is fragmented or sheared, ligated with Ion-specific adapter sequences, and then size-selected for the library fragments of the desired length.

Amplicon libraries are constructed from PCR-amplified DNA fragments and are used for targeted sequencing (e.g., investigating variants at known genomic locations). There are two types of amplicon libraries, short and long.

A short amplicon library contains DNA fragments (targets) with lengths that are compatible with the Ion template preparation kits without any further shearing or fragmentation during library preparation. Additionally, no size-selection step is required, as the amplicons are already within the desired size range.

A long amplicon library contains DNA fragments (targets) with lengths that are longer than those compatible with the Ion template kits and requires further shearing or fragmentation during library preparation. The library preparation protocol for long amplicons is similar to fragment libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What do I need to do to prep the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

When handling barcode adapters, be careful to avoid cross-contamination by changing gloves frequently and by carefully removing the plate seal.
- Pulse vortex the IonCode Barcode Adapters plate several times.
- Centrifuge the IonCode Barcode Adapters plate for 1 minute at 1000 x g to collect contents.
- Carefully remove, then discard the plate seal.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many reactions are possible with the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The plate consists of 960 reactions in total with 10 reactions for each of the 96 barcodes.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

In what format are the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The barcodes are pre-diluted, ready-to-use. The 96-well plate format allows multichannel pipetting for more efficient work.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the shipping and storage conditions for IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit is shipped on dry ice and we recommend storing at -30 degrees C to -5 degrees C in an upright position.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

View all Ion AmpliSeq™ Kit for Chef DL8 FAQs