PureLink™ Microbiome DNA Purification Kit
PureLink™ Microbiome DNA Purification Kit
Invitrogen™

PureLink™ Microbiome DNA Purification Kit

The PureLink™ Microbiome DNA Purification Kit enables fast purification of high-quality microbial and host DNA from a wide variety ofRead more
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Catalog number A29790
Price (USD)
400.00
Each
Add to cart
Price (USD)
400.00
Each
Add to cart
The PureLink™ Microbiome DNA Purification Kit enables fast purification of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil. The kit uses proven PureLink spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing, or other applications. The highly efficient triple lysis approach, fast removal of inhibitors, and versatility make this the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.

Features of the PureLink Microbiome DNA Purification Kit include:

• Efficient lysis of all microorganisms (including durable species with thicker and more complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
• Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
• Streamlined protocols for numerous biological samples
• Recovery of high-purity DNA compatible with common downstream applications such as qPCR and next-generation sequencing

One kit to isolate microbial and host DNA from a diverse set of sample types
The PureLink Microbiome DNA Purification Kit minimizes the need to order individual kits for specific sample types because it has been optimized for use with a wide range of biological samples.

The kit enables microbial—and, where applicable, host—DNA purification from the following samples:

• Stool
• Urine
• Saliva
• Swabs (vaginal, buccal, skin, rectal, environmental)
• Transport media
• Growth media
• Soil

Analyzing the human microbiome
The human body is populated by 100 trillion bacteria, archaea, fungi, protists, and viruses, all of which play a fundamental role in our well-being. The term 'microbiome' refers to the various different microbes in those communities. Presently, their genes are the most straightforward method of identifying these organisms. Deviations from healthy microbial compositions have been linked to many human diseases, including inflammatory bowel disease, obesity, cancer, asthma, diabetes, and allergies.

Purification of the aggregate microbial DNA in a biological sample is a key step in understanding how the microbiome influences human health. It is of utmost importance that the DNA recovered accurately reflects the representation of the diverse microbes in the sample community. The mechanical disruption and triple lysis approach used in the PureLink Microbiome DNA Kit help ensure that microbial DNA from even the toughest samples, such as gram-positive bacteria found in stool, can be recovered—allowing you to identify the full spectrum of microbes present.
For research use only. Not for use in diagnostic procedures.
Specifications
Column TypeSpin Column
Isolation TechnologySilica Spin Column
Sample TypeMicrobial Culture, Saliva, Soil, Stool, Host DNA from Stool, Swabs (Buccal, Vaginal, Skin, Rectal, or Environmental), Cells, Food and Environmental
ScaleMini
Elution Volume50 to 200 μL
Final Product TypeMicrobial and host DNA
For Use With (Application)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Quantity50 Preps
Shipping ConditionRoom Temperature
Test TimeUp to 55 min. (Including sample homogenization)
Yield≤25 μg
Starting Material AmountBuccal/Vaginal/Skin/Rectal/Environmental Swab: 1 swab
Microbial Culture: ≤5 mL
Saliva: ≤2 mL
Soil: ≤2 g
Stool: ≤2 g
Urine: ≤10 mL
Unit SizeEach
Contents & Storage
• 40 mL S1 Lysis Buffer; room temperature
• 5 mL S2 Lysis Enhancer; room temperature
• 12.5 mL S3 Cleanup Buffer; room temperature
• 45 mL S4 Binding Buffer; room temperature
• 13 mL S5 Wash Buffer (concentrated); room temperature
• 5 mL S6 Elution Buffer; room temperature
• 50 PureLink Columns with Collection Tubes; room temperature
• 100 PureLink Collection Tubes; room temperature
• 50 Bead Tubes; room temperature

Frequently asked questions (FAQs)

I am using the PureLink Microbiome DNA Purification Kit (Cat. No. A29790). When should I remove the swab?

The swab should be removed after incubation, prior to bead beating.

Does the elution buffer in the PureLink Microbiome DNA Purification Kit (Cat. No. A29790) contain EDTA?

Yes, the elution buffer in the PureLink Microbiome DNA Purification Kit contains EDTA. An alternative that does not contain EDTA is Tris Buffer (10 mM Tris-HCl, pH 8.0-9.0). Eluting in water is possible, but the low pH of water could lead to reduced stability and less accurate OD readings.

When using the PureLink Microbiome DNA Purification Kit with soil samples, I am getting less than 400 µL supernatant after lysis. Can you provide me with any suggestions?

For some samples, including soil and stool samples, it can be difficult to withdraw 400 µL supernatant while avoiding debris. If less than 250 µL of supernatant is transferred, add S1-Lysis buffer to bring the volume to 400 µL, before adding S3-Cleanup Buffer.

I am getting low yield using PureLink Microbiome DNA Purification Kit. How can I improve the yield?

Low yield could be caused by inefficient lysis and/or low levels of DNA in the sample. Please try heating samples at 95 degrees C for 5-10 minutes instead of 65 degrees C for 10 minutes after adding S2-Lysis Enhancer. Bead beat for a longer time or at a higher power setting. You can also try to perform the experiment with more starting material, but do not exceed what we specify in the protocol. However for some challenging samples, too much starting material can also result in low yield. In this case, please try less starting material and/or increase the volume of S1-Lysis Buffer.

There are inhibitors in the isolated DNA from environmental samples using PureLink Microbiome DNA Purification Kit. Is there a way to rescue it and obtain good PCR results?

There are several options to rescue your DNA obtain good PCR results including diluting your DNA sample, using specific enzymes, and/or using an additive. The methods are described below:

- Dilute your DNA sample 10-100 times prior to PCR which will dilute out the contaminants while the PCR signal in many cases will remain strong.
- Use “tough” enzymes that were specifically designed and optimized to be effective in harsh environments, and work in the presence of high levels of inhibitors, for instance, TaqPath qPCR Master Mix, CG Reagent.
- Use a BSA additive for the PCR reaction. In most cases, if used at appropriate concentration, BSA can rescue your sample.

You can also try repeating the purification with less starting material or increased volume of S1-Lysis buffer. Additionally, after the addition of the S3- Cleanup buffer, you can try incubating the sample for 10 minutes on ice before centrifugation.

View all PureLink™ Microbiome DNA Purification Kit FAQs