Precision ID GlobalFiler™ NGS STR Panel v2

The Applied Biosystems Precision ID GlobalFiler NGS STR Panel v2 enables an innovative next-generation sequencing (NGS) approach to analysis of DNA mixtures for casework applications using massively parallel sequencing built on Ion AmpliSeq technology for superior efficiency. The panel includes 35 markers comprised of 21 CODIS STR markers, 9 additional multi-allelic STR markers, and 4 sex determination markers to aid in mixture interpretation of complex casework samples.

Using a minimal DNA input of 125 pg, this targeted forensic marker panel is optimized for use with the Precision ID DL8 Kit (Cat. No. A33212) and the Ion S5 Precision ID Chef & Sequencing kit (Cat. No. A33208). The automated Precision ID workflow enables streamlined sample extraction-to-genotypes using the Ion Chef System, Ion GeneStudio series sequencer, and Converge Software NGS Analysis Module.

Simplicity
• As little as 125 pg of input DNA
• Manual and automated Ion Chef library preparation
• Template preparation with the Ion Chef System
• Interpret profiles with the Converge NGS Analysis Module

Scalability
• Ion 520 or Ion 530 chip-enabled
• Ability to multiplex up to 32 samples for efficient sample processing

The Converge Software NGS module offers complete NGS data analysis of targets amplified with the Precision ID GlobalFiler NGS STR Panel v2. STR markers are displayed as both allele number and base sequence for each repeat structure, offering enhanced mixture resolution. The software also analyzes single nucleotide polymorphisms (SNPs) in flanking regions of the STR markers. This unique NGS STR panel combines maximum compatibility with CODIS core loci databasing standards and superior discrimination power, enabling forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The Precision ID GlobalFiler NGS STR Panel v2 is optimized for casework samples.

Compatible with the Ion S5/S5XL and Ion GeneStudio series systems only. Not for use with the Ion OneTouch2 System or Ion PGM System.

NOTE: The GlobalFiler NGS STR Panel v2 provides sufficient material to perform 96 reactions using the manual Precision ID Library kit (Cat. No. A26435) or 32 reactions using the automated Precision ID DL8 Library Kit (Cat. No. A33212).