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PureLink™ Fast Low-Endotoxin Maxi Plasmid Purification Kit
The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfectionRead more
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| Catalog Number | No. of Reactions |
|---|---|
| A36228 | 20 Preps |
| A35895 | 10 Preps |
Catalog number A36228
Price (USD)
626.00
Each
No. of Reactions:
20 Preps
Price (USD)
626.00
Each
The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (less than 1 EU/μg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 1.5 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.
NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold.
Advantages of the PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit include:
• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/μg endotoxins
• High yields—isolate up to 1.5 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold.
Advantages of the PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit include:
• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/μg endotoxins
• High yields—isolate up to 1.5 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
Isolation TechnologySilica Column
ScaleMaxi
For Use With (Application)Cloning, PCR
No. of Reactions20 Preps
Prep ScaleMaxi input culture of 100-500 mL to obtain a yield of up to 850 μg
Product LinePureLink™
Product TypePlasmid Purification Kit
Quantity20 Preps
Shipping ConditionRoom Temperature
Test Time30 min.
Unit SizeEach
Contents & Storage
• Resuspension buffer, store at 4°C upon reciept
• Lysis buffer, store at room temperature
• Precipitation buffer, store at room temperature
• Binding buffer, store at room temperature
• Wash buffer 1, store at room temperature
• Wash buffer 2 (concentrate), store at room temperature
• Elution buffer, store at room temperature
• Column assemblies, store at room temperature
• Syringe filters, store at room temperature
• Syringe plungers, store at room temperature
• Collection tubes, store at room temperature
• Lysis buffer, store at room temperature
• Precipitation buffer, store at room temperature
• Binding buffer, store at room temperature
• Wash buffer 1, store at room temperature
• Wash buffer 2 (concentrate), store at room temperature
• Elution buffer, store at room temperature
• Column assemblies, store at room temperature
• Syringe filters, store at room temperature
• Syringe plungers, store at room temperature
• Collection tubes, store at room temperature