Primary Rat Hippocampal Neurons, Sprague Dawley
Increase cell survival by more than 50% with new Gibco™ B-27™ Plus Neuronal Culture System.
Primary Rat Hippocampal Neurons, Sprague Dawley
Gibco™

Primary Rat Hippocampal Neurons, Sprague Dawley

Gibco Primary Rat Hippocampal Neurons, Sprague Dawley, are isolated and cryopreserved from Sprague Dawley embryonic day-18 rats. A flexible, ready-to-use,Read more
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Catalog number A36513
Price (USD)
796.00
Each
Add to cart
Price (USD)
796.00
Each
Add to cart
Gibco Primary Rat Hippocampal Neurons, Sprague Dawley, are isolated and cryopreserved from Sprague Dawley embryonic day-18 rats. A flexible, ready-to-use, and quality alternative to fresh neurons, each vial contains one million cells per mL. These neurons consistently yield post-thaw viability in the range of 50–80%, have high neuronal purity, and are validated for functionality.

• High viability: post-thaw viability of 50% or greater
• High purity: greater than 70% neuronal phenotype
• Functionally validated: tested for spontaneous synchronous calcium flux and synaptic release activity

For Research Use Only. Not for use in diagnostic procedures.
Specifications
AgeFetal
Cell TypeNeural Cells
Culture TypeMammalian Cell Culture
FormCryopreserved
SpeciesRat
TissueHippocampus
No. of Cells1 x 106
Product TypeRat Hippocampus Neuron
Tested ForCell Purity, Sterility, Viability, Mycoplasma
Unit SizeEach
Contents & Storage
Store in liquid nitrogen, vapor-phase.

Frequently asked questions (FAQs)

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the purpose of L-glutamate in the media for primary hippocampal neurons?

Glutamate helps hippocampal neurons sprout and promotes viability. However, L-glutamate can only be supplemented in the medium up to day 4 in culture because it is toxic to mature neurons.

Can primary rat neurons be cryopreserved again after they are cultured?

Primary rat neurons cannot be cryopreserved again because they will not revive.

Can primary rat neurons proliferate?

No, primary rat neurons do not proliferate.

View all Primary Rat Hippocampal Neurons, Sprague Dawley FAQs