Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems
Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems
Invitrogen™

Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems

The Invitrogen Collibri PCR-free ES DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberProduct TypeNo. of Reactions
A38602024PCR-free ES DNA Library Prep Kit with UD Indexes (Set A, 1-24)24 Preps
A38545024WPCR-free ES DNA Library Prep Core Kit24 Preps
A38603096WPCR-free ES DNA Library Prep Core Kit96 Preps
A38545024PCR-free ES DNA Library Prep Kit with CD Indexes24 Preps
A38603096PCR-free ES DNA Library Prep Kit with CD Indexes96 Preps
A43602024PCR-free ES DNA Library Prep Kit with UD Indexes (Set B, 25-48)24 Preps
A43603024PCR-free ES DNA Library Prep Kit with UD Indexes (Set C, 49-72)24 Preps
A43604024PCR-free ES DNA Library Prep Kit with UD Indexes (Set D, 73-96)24 Preps
A38608196PCR-free ES DNA Library Prep Kit with UD Indexes (Set A-D, 1-96)96 Preps
Catalog number A38602024
Price (USD)
1,198.00
Each
Add to cart
Product Type:
PCR-free ES DNA Library Prep Kit with UD Indexes (Set A, 1-24)
No. of Reactions:
24 Preps
Request bulk or custom format
Price (USD)
1,198.00
Each
Add to cart

The Collibri PCR-free ES DNA Library Preparation Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about two hours for the PCR-free workflow. If needed, Collibri Library Amplification Master Mix with primers can be bought separately. Or see the Collibri ES DNA Library Preparation Kit, designed for fast and convenient library preparation with PCR from a wide range of inputs (1–500 ng).

The Collibri PCR-free ES DNA Library Preparation Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about two hours for the PCR-free workflow. If needed, Collibri Library Amplification Master Mix with primers can be bought separately. Or see the Collibri ES DNA Library Preparation Kit, designed for fast and convenient library preparation with PCR from a wide range of inputs (1–500 ng).

The Collibri PCR-free ES DNA Library Preparation Kit:
• Includes all necessary components in a single kit* for quick use
• Is empowered on miniaturization liquid handlers to cut on costs
• Provides in-process visual feedback for easier handling
•Is suitable for conventional automation to increase throughput

* The kit consists of enzymatic modification, size selection/clean-up, and indexed adapter-containing modules, except for the Core kit, which enables Collibri combination with desired indexed adapters

The Collibri PCR-free ES DNA Library Preparation Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. Moreover, the enzymatic shearing (ES) module of the kit generates a consistently high yield of library, which is enough for library quantification  and sequencing and multiple runs on Illumina instruments.

Applications
The Collibri PCR-free ES DNA Library Preparation Kit is recommended for:
• Whole genome sequencing
• Resequencing
• De novo assembly

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionThe product contains modules A38575024, A38587024, A43190024
FormatKit
Sample TypeDNA
Sequencing TypeGenome and DNA Sequencing
For Use With (Equipment)HiSeq X, HiSeq2500, HiSeq3000, HiSeq4000, MiSeq or iSeq, NextSeq 550, NovaSeq 6000
For Use With (Application)Whole Genome Sequencing, Resequencing, De Novo Assembly
LibrariesFragment Library
No. of Reactions24 Preps
Product LineCollibri™
Product TypePCR-free ES DNA Library Prep Kit with UD Indexes (Set A, 1-24)
Quantity24 preps
Shipping ConditionDry Ice
SpeciesAll
Workflow StepLibrary Generation
Unit SizeEach
Contents & Storage
• Collibri PCR-free ES DNA Library Prep Kit, store at -20°C
• Collibri DNA Library Cleanup Kit, store at 4°C
• Collibri DNA UD Indexes, store at -20°C

Frequently asked questions (FAQs)

Can indexed adaptors or other modules be purchased separately for the Collibri DNA Library Prep kits?

Indexed adapters and other modules in the kit can be purchased separately. Please refer to thermofisher.com/collibriDNA and contact us for more details.

With the Collibri DNA Library Prep kits, what is the distribution of enzymatically sheared DNA in the library?

Fragment size ranges from 150 bp to 850 bp. For optimal results, we recommend and provide detailed instructions for 350 bp and 550 bp average size library construction.

With the Collibri DNA Library Prep kits, can I use indexed adaptors interchangeably between RNA-seq and DNA-seq applications?

No. They are specifically designed for a product and cannot be used interchangeably.

With the Collibri DNA Library Prep kits, why is my ligation master mix orange instead of red?

Dyes inside the master mix react with CO2 and change the color from red to orange. The mix is still usable when the color changes to orange. To prevent discoloration, avoid keeping it on dry ice.

Are Collibri DNA Library Prep kits compatible with FFPE samples?

Collibri PS DNA Library Prep Kit can be used for library construction from lower-quality DNA (such as FFPE samples). However, the yields of adaptor-ligated molecules are typically lower compared to the yields obtained with high-quality DNA.

Collibri ES DNA Library Prep Kit is not recommended for library construction from FFPE samples.

View all Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems FAQs