EasyPep™ MS Sample Prep Kits
EasyPep™ MS Sample Prep Kits
Thermo Scientific™

EasyPep™ MS Sample Prep Kits

Generate high quality proteomic samples for MS analysis in less than four hours using optimized EasyPep MS Sample Prep kits.
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Catalog NumberDescription
A40006Mini MS Sample Prep Kit
A57864Micro MS Sample Prep Kit
A45733MS Sample Prep Kit
A45734Maxi Sample Prep Kit
A45735Lysis Buffer
A57865Peptide Clean-up Plate
Catalog number A40006
Price (USD)
672.00
Each
Add to cart
Description:
Mini MS Sample Prep Kit
Price (USD)
672.00
Each
Add to cart

EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than three hours. Kits are optimized to process protein samples from 10–100 μg with a high yield of MS-ready peptides.

• Complete—includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples
• Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
• Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
• Time-saving—sample processing time reduced from more than one day to less than three hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling

The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionMini MS Sample Prep Kit
For Use With (Equipment)Mass Spectrometer
Product TypeSample Preparation Kit
Product LineEasyPep™
Shipping ConditionWet Ice
Starting MaterialProtein Samples
Final Product TypePeptides
Detection MethodMass Spectrometry
Workflow StepCell Lysis
Quantity20 Reactions
Unit SizeEach

Frequently asked questions (FAQs)

How can I determine peptide yield in my mass spectrometry samples after sample clean-up?

Peptide yield can be measured using the Pierce Quantitative Colorimetric Peptide Assay (Cat. No. 23275) or the Pierce Quantitative Fluorometric Peptide Assay (Cat. No. 23290). The choice of peptide assay depends on the sample type and composition of the sample buffer. The fluorometric peptide assay cannot be used to measure peptides with chemically modified amines such as acetylated peptides or TMT-labeled protein digests. The colorimetric assay can measure a wider range of samples but is not as sensitive as the fluorometric assay, requiring more sample for accurate detection. Finally, both assays are susceptible to interfering compounds in the sample or buffer which should be avoided or removed for best results.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I have good peptide identification numbers but the variation between sample replicates is high. What do you recommend to improve sample reproducibility?

We recommend reviewing your sample-prep workflow to ensure consistent protein extraction, reduction/alkylation, digestion, and clean-up. We recommend using EasyPep products for high quality, reproducible sample preparation (EasyPep Maxi Sample Prep Kit, EasyPep Mini MS Sample Prep Kit, EasyPep 96 MS Sample Prep Kit). We also recommend quantifying peptides using the Pierce Quantitative Fluorometric Peptide Assay (Cat. No. 23290) or Pierce Quantitative Coloimetric Peptide Assay (Cat. No. 23275) to ensure that the same amount of peptides are being used for each LC-MS analysis. Poor reproducibility could also be related to the LC-MS system performance which may require recalibration using Pierce Calibration Solutions. System performance can be assessed using protein digest standards such as Pierce HeLa Protein Digest Standard or Pierce TMT11plex Yeast Digest Standard and peptide standards such as Pierce Peptide Retention Time Calibration Mixture or Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.