CTS™ LV-MAX™ Production Medium
CTS™ LV-MAX™ Production Medium
Gibco™

CTS™ LV-MAX™ Production Medium

Gibco CTS LV-MAX Production Medium is a chemically-defined, serum-free, protein-free medium validated for recombinant lentiviral vector production. In addition toRead more
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Catalog NumberQuantity
A41240046 x 1 L
A41240011 L
A412400210 L
A412400320 L
Catalog number A4124004
Price (USD)
1,942.00
Each
Add to cart
Quantity:
6 x 1 L
Price (USD)
1,942.00
Each
Add to cart

Gibco CTS LV-MAX Production Medium is a chemically-defined, serum-free, protein-free medium validated for recombinant lentiviral vector production. In addition to supporting high cell density suspension cultures of CTS Viral Production Cells (HEK293F-derived cells), this medium is suitable for preclinical-, clinical-, and commercial-scale transient transfection and lentiviral production when used with the CTS LV-MAX Transfection Kit. CTS LV-MAX Production Medium undergoes stringent QC testing to ensure it is appropriate for the manufacturing of cell and gene therapy applications.

CTS LV-MAX Production Medium may be applicable for other recombinant viral vector production that uses a HEK293 medium.

CTS LV-MAX Production Medium features include:

  • Supports CTS Viral Production Cells to achieve densities of over 1 x 107 cells/mL with minimal cell clumping
  • Enables high viral titers of up to 1 x 108 TU/mL (unconcentrated lentiviral vectors–GFP)
  • Scalable protocol for 96-deep well blocks, flasks, and bioreactors
  • Regulatory-compliant medium manufactured in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to the ISO 13485. Drug Master File and Regulatory Support File available upon request.

CTS LV-MAX Production Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. The medium contains no human or animal-origin components. It is not recommended for adherent cell cultures. The formulation of this product is identical to research-grade LV-MAX Production Medium, but CTS LV-MAX Production Medium is validated for clinical and commercial therapeutic applications.

Custom configuration and packaging available upon request.

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.
Specifications
Cell LineViral Production Cells
Cell TypeHEK293
ClassificationAnimal Origin-free
FormLiquid
Product TypeLV Production Medium
Shelf Life24 months from date of manufacture
SterilitySterile-filtered
FormatBottle
For Use With (Application)LV Production
Manufacturing QualityISO 13485, MDSAP, FDA-registered, 21 CFR 820
Quantity6 x 1 L
Shipping ConditionAmbient
Unit SizeEach
Contents & Storage
6 x 1 L bottles. Store at 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

Are CTS Viral Production Cells 293T-derived?

No, CTS Viral Production Cells are HEK 293F suspension cells that have been adapted to CTS LV-MAX Production Medium. They do not express the SV40 large T antigen.

How do I bank CTS Viral Production Cells?

CTS Viral Production Cells can be frozen directly in cryogenic medium consisting of 90% CTS LV-MAX Production Medium plus 10% DMSO. Here is the protocol:

- Bank cells at passage 3 when cell density reaches ˜3.0-4.5 x 10E6 viable cells/mL at >95% viability.
- Centrifuge the cells at 100 x g (low speed) for 5 mins to pellet. Discard the spent medium.
- Prepare freezing medium: e.g., 10 mL of freezing medium is 9 mL of CTS LV-MAX Production Medium + 1 mL DMSO; mix well and sterilize by 0.2 µm filtration.
- Resuspend the cell pellet in the prepared freezing medium.
- Dilute the cells to a final density of 10 x10E6 viable cells/mL and aliquot 1 mL per cryovial.
- Freeze the cells at -80 degrees C for 1 day.
- Transfer frozen vials to liquid nitrogen for long-term storage.

Why do you recommend using V bottom 96-well plates for harvesting lentivirus from CTS Viral Production Cells?

We recommend using V-bottom 96-well plates to harvest the supernatant containing lentiviral particles as they allow for easier detection of the cell pellets after centrifugation of the cultures.

During lentivirus production from CTS Viral Production Cells, will the shaker vibration affect the cell growth of other cultures maintained in the same incubator or in stacked incubators?

We recommend maintaining shaking cultures in a separate incubator from all other incubators to avoid vibration effects. Alternatively, we recommend using a small, quiet shaker such as the Thermo Scientific CO2 Resistant Shaker (Cat. No. 88881102) for convenience and best performance.

Is 8% CO2 a growth requirement for CTS Viral Production Cells?

We generally recommend culturing the CTS Viral Production Cells under 8% CO2 conditions. We found that cells cultured in 5% CO2 grew slower and were more prone to more cell clumping than when grown in 8% CO2.

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