BenchStable™ DMEM
BenchStable™ DMEM
Gibco™

BenchStable™ DMEM

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Gibco DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalianRead more
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Catalog NumberQuantity
A4192101500 mL
A419210210 x 500 mL
Catalog number A4192101
Price (USD)
44.90
500 mL
Add to cart
Quantity:
500 mL
Price (USD)
44.90
500 mL
Add to cart

Gibco DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

BenchStable DMEM has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional DMEM when supplemented with ≥10% fetal bovine serum (FBS).

This DMEM is modified as follows:
• Optimized for room temperature stability
• With High Glucose, GlutaMAX, Phenol Red
• Without Sodium Pyruvate

Using BenchStable DMEM
DMEM is unique from other media as it contains four times the concentration of amino acids and vitamins as the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and so requires a 5–10% CO2 environment to maintain physiological pH. DMEM with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. BenchStable DMEM contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineHeLa, 293, Cos-7, and PC-12
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Serum LevelStandard Serum Supplementation
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesHigh Glucose, GlutaMAX, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Green FeaturesEnergy efficient, Fewer resources used, Sustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 9001: 2015 standard
Product LineBenchStable™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit Size500 mL
Contents & Storage
Store at room temperature. Protect from light.

Frequently asked questions (FAQs)

Is it okay if I supplement BenchStable media with more than 10% FBS?

We recommended supplementing BenchStable media with at least 10% FBS for best results. If your culture method calls for greater than 10% FBS supplementation using our other existing media, we recommend that you use the same concentration with BenchStable media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it okay if I supplement BenchStable media with 5% FBS?

We recommend supplementing BenchStable media with at least 10% FBS for best results. We have run tests on limited cell types using 5% FBS concentration and have not observed compromised performance. Please contact Tech Support at techsupport@thermofisher.com to ensure that your specific application allows for 5% serum supplementation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which cell lines have been tested in BenchStable media?

We have tested our BenchStable media for growth and morphology on the following cell lines: A549, CHO-K1, HEK293, HeLa, HL-60, Jurkat, MCF7, MDA-MB-231, Neuro2A, SH-SY5Y, and THP-1.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer BenchStable media without phenol red?

At this time, we do not offer BenchStable media without phenol red.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the shelf-life of BenchStable media improve if stored at lower temperature (2-8 degrees C)?

The shelf life of BenchStable media is unchanged when stored cold (2-4 degrees C) or ambient (15-30 degrees C).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.