Oncomine Focus Assay, Chef-Ready Library
Oncomine Focus Assay, Chef-Ready Library
Ion Torrent™

Oncomine Focus Assay, Chef-Ready Library

The Oncomine Focus Assay is a targeted next-generation sequencing (NGS), multi-biomarker assay that enables you to detect variants across 52Read more
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Catalog number A42008
Price (USD)
13,180.00
Each
Add to cart
Price (USD)
13,180.00
Each
Add to cart
The Oncomine Focus Assay is a targeted next-generation sequencing (NGS), multi-biomarker assay that enables you to detect variants across 52 cancer-relevant genes from DNA and RNA in a single workflow. It provides reagents for library construction and two pools—one DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per primer pool, the assay enables analysis of hotpots, SNVs, indels, CNVs, and gene fusions in a single workflow. For a kit that includes reagents for manual library preparation, please see the Oncomine Focus Assay, AmpliSeq Library.

The Oncomine Focus Assay, Chef-Ready Library, is for the Ion Chef System and Ion GeneStudio S5 System workflow. It contains all the reagents needed to prepare libraries for 32 samples:

• Oncomine Focus Assay, DNA Chef-Ready panel—contains the DNA primer pool
• Oncomine Focus Assay, RNA Chef-Ready panel—contains the RNA primer pool
• Ion AmpliSeq Kit for Chef DL8 reagents—allows you to automate library prep on the Ion Chef System

From sample to answer
The Oncomine Focus Assay is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.Learn more about Oncomine Focus Assays and Oncomine Oncology.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Site-Directed Mutagenesis
LibrariesTargeted Sequencing Library
No. of Samples32 samples
Product LineOncomine™
Product TypeFocus Assay
Shipping ConditionApproved for shipment at Room Temperature or on Wet or Dry Ice
Workflow StepLibrary Generation
Unit SizeEach
Contents & Storage
• 1 x Oncomine Focus Assay, DNA Chef-Ready panel (2X)
• 1 x Oncomine Focus Assay, RNA Chef-Ready panel (2X)
• 2 x Ion AmpliSeq Kit for Chef DL8 reagents

Note: SuperScript transcriptase for cDNA synthesis not included. Must be purchased separately.

Frequently asked questions (FAQs)

With the Oncomine Focus Assay, Chef-Ready Library kit, where can I find the combined barcoded AmpliSeq libraries when the run is complete?

After the completion of a run, the Ion Chef Instrument holds the barcoded libraries in the tube loaded in Position D of the Reagents cartridge. To avoid fluid loss due to evaporation, remove and cap the tube of combined barcoded libraries as soon as possible after run completion. Do not leave the tube in the instrument longer than 24 hours after the start of the run. After 24 hours from the start of the run, the instrument chiller will stop actively cooling, and the sample will be held at 27 degrees C.

The tube in Position D of the Ion AmpliSeq Chef Reagents DL8 Cartridge will contain 700 µL of combined barcoded libraries at completion of the run. The libraries are at >>100 pM (total combined library concentration) and are ready to use in template preparation. One can store unused portions of combined libraries at 4 to 8 degrees C for up to 1 month. For longer-term storage, store at -30 to -10 degrees C.

With the Oncomine Focus Assay, Chef-Ready Library kit, can I process fewer than 8 samples per Ion AmpliSeq run on the Ion Chef System?

Yes, fewer than 8 samples may be processed per run but keep in mind that a run consumes kit reagents for 8 samples regardless of the sample number.

If I process less than 8 samples using the Oncomine Focus Assay, Chef-Ready Library kit, do I need to quantitate the final combined output library?

If you are processing ≤5 samples, we recommend that you quantify your output combined library by qPCR to ensure that an optimal concentration is used in templating reactions.

If you are processing fewer than 8 samples, it is preferable to add replicates or positive control samples to the run. Otherwise, pipet 15 µL of nuclease-free water as non-template control into column 1 wells that do not contain a DNA sample.

What is the RNA input for the Oncomine Focus Assay, Chef-Ready Library kit?

Each reverse transcription reaction requires 10 ng of DNase-treated total RNA (≥1.43 ng/µL).

What is the DNA input for the Oncomine Focus Assay, Chef-Ready Library kit?

For each target amplification reaction, use 300-30,000 copies of DNA (10 ng of mammalian gDNA) from normal or FFPE tissue.

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