E-Gel™ Double Comb Agarose Gels with SYBR™ Safe DNA Gel Stain
E-Gel™ Double Comb Agarose Gels with SYBR™ Safe DNA Gel Stain
Invitrogen™

E-Gel™ Double Comb Agarose Gels with SYBR™ Safe DNA Gel Stain

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Invitrogen E-Gel Double Comb Agarose Gels with SYBR Safe DNA Gel Stain are designed for fast, convenient, and less-hazardous DNA sample electrophoresis.
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Catalog NumberGel PercentageSeparation RangeQuantity
A423482%50 to 2000 bp10 Gels
A423471%100 to 5000 bp10 Gels
A448851%100 to 5000 bp2 x 10 Gels
A448861%100 to 5000 bp5 x 10 Gels
A423902%50 to 2000 bp2 x 10 Gels
A448842%50 to 2000 bp5 x 10 Gels
Catalog number A42348
Price (USD)
240.00
Each
Add to cart
Gel Percentage:
2%
Separation Range:
50 to 2000 bp
Quantity:
10 Gels
Price (USD)
240.00
Each
Add to cart
Invitrogen E-Gel Double Comb Agarose Gels with SYBR Safe DNA Gel Stain are designed for fast, convenient, and less-hazardous DNA sample electrophoresis. Each E-Gel Double Comb gel cassette contains all components and stain required for efficient gel separation and analysis–just load your samples and run. E-Gel Double Comb gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, and genotyping samples.

Features of E-Gel Double Comb Agarose Gels include:

  • Instantaneous setup—ready for use when you are and no gel preparation or additional buffers required
  • Less-hazardous choice—contains SYBR Safe DNA stain, a less-hazardous alternative to ethidium bromide
  • High performance—detect 3 ng of sample DNA with a separation time as short as 13–15 minutes
  • In-process control—view DNA sample migration in real time
  • Easy sample access—openable cassette design allows easy in-gel access for convenient DNA sample excision or transfer to membranes
  • Better sample integrity—exploits blue-light excitation wavelength to minimize DNA damage, as opposed to UV

Less hazardous option

Choose SYBR Safe DNA gel stain to minimize exposure to hazardous and mutagenic options such as ethidium bromide and UV light. Precast E-Gel Double Comb gels with SYBR Safe stain, in combination with blue-light trans-illumination, offer better sample integrity and a less hazardous work environment.

Fast and convenient analysis

E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel Double Comb gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not require any gel or buffer preparation or gel staining steps. Just load your samples and run.

Live monitoring of DNA migration

E-Gel Double Comb gels include SYBR Safe DNA Gel Stain, which has an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, the gels enable less hazardous real-time monitoring of DNA migration without risk to the eyes or risk of damaging the sample, as opposed to UV visualization.

Easy access to the gel

E-Gel Double Comb cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for southern blot analysis.

Running and imaging devices

E-Gel Double Comb gels require the E-Gel Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel agarose gel separation and analysis.

Available in a variety of gel formats

E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The 22-well E-Gel Double Comb gels with SYBR Safe stain are available in 1% and 2% gel percentages.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible LaddersE-Gel™ 1 Kb Plus Express Ladder
Gel Percentage2%
Gel TypeE-Gel
Product TypeAgarose Gel
Separation Range50 to 2000 bp
Stain TypeSYBR™ Safe
Wells22-well
Green FeaturesLess hazardous, less waste, fewer resources used
Quantity10 Gels
Well DesignDouble Comb
Unit SizeEach
Contents & Storage
• Each box contains 10 gels

Store strictly at room temperature. Do not freeze.
Avoid cold spots near freezers or other uncontrolled environments.

Frequently asked questions (FAQs)

How can I view DNA on my E-Gel agarose gel with SYBR Safe DNA stain?

SYBR Safe-stained gels can be visualized with a standard UV transilluminator, a laser-based scanner equipped with an excitation source in the UV range or between 470 and 530 nm, or a blue-light transilluminator.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

My E-Gel agarose gel has speckles when viewed in the imager. What should I do?

Here are some suggestions:

- Try cleaning the cassettes with alcohol and Kimwipes wipers.
- Try cleaning the camera lens.
- Try to adjust the exposure time and brightness options of the documentation system you are using.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

My sample is leaking from the wells when running my E-Gel agarose gels. What happened?

Please ensure that you have not overloaded the well and that the wells were not damaged during comb removal.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

I accidentally stored my E-Gel agarose gels at 4 degrees C instead of room temperature. Can I still use them?

While we recommend storage at room temperature, these gels will still be usable. Bring the gels to room temperature prior to the run for optimal conditions.

Find additional tips, troubleshooting help, and resources within ourNucleic Acid Gel Electrophoresis and Blotting Support Center.

What loading buffer should I use for my E-Gel agarose gels?

Loading buffer is optional. Samples can be loaded directly into the wells if no buffer is used, or you can dilute them with deionized water or TE buffer. If you want to use a loading buffer, please see the recipes below:

E-Gel agarose gels (including EX)
10 mM Tris-HCl, pH 7.5
1 mM EDTA
0.005% bromophenol blue
0.005% xylene cyanol FF
E-Gel CloneWell II and E-Gel SizeSelect II agarose gels
10 mM Tris-HCl, pH 7.5
1 mM EDTA

Alternatively, you can use 10X BlueJuice Gel Loading Buffer or TrackIt Loading Buffer. Dilute this buffer 50- to 200-fold to obtain optimal results with E-Gel agarose gels.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.