iBright™ CL750 Imaging System
iBright™ CL750 Imaging System
Invitrogen™

iBright™ CL750 Imaging System

Capture western blot and gel images and analyze data efficiently and easily with the Invitrogen iBright CL750 Imaging System. ThisRead more
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Catalog number A44116
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Capture western blot and gel images and analyze data efficiently and easily with the Invitrogen iBright CL750 Imaging System. This system supports basic imaging applications associated with chemiluminescent western blots, as well as fluorescent stained nucleic acid gels, fluorescent stained protein gels, and colorimetric stained protein gels.

For expanded functionality, we also offer the more powerful iBright CL1500 Imaging System and iBright FL1500 Imaging System. The iBright CL1500 Imaging System supports the main imaging applications associated with chemiluminescent and colorimetric western blots, as well as fluorescent stained nucleic acid gels, fluorescent stained protein gels, colorimetric stained protein gels, and colorimetric membrane stains. The iBright FL1500 Imaging System features the same imaging capabilities as the iBright CL1500 Imaging System, but also offers fluorescent western blot imaging capability.

Explore all models and features of the iBright Imaging System ›

Features of the iBright CL750 Imaging System:
9.1 MP cooled CCD camera—high sensitivity and dynamic range to help enable the detection of subtle differences in samples
Smart Exposure technology—provides rapid determination of optimal exposure time to help minimize the need to repeat exposures to acquire the desired signal
High Dynamic Range imaging*—Smart Range HDR combines optimized short and long exposures into a single HDR image to capture an extended linear dynamic range and providing high-quality non-saturated data for improved quantitation
Simple interface—clear layout of functions and features combined with a 12.1-inch capacitive touchscreen for a smooth imaging experience
22.5 x 18.0-cm field of view—large field of view for high-throughput imaging (image up to four mini or two midi blots at a time)
Green LED-based transilluminator—effectively excite popular DNA dyes such as ethidium bromide and SYBR Green dyes with an alternative to UV-based transilluminators
Expanded imaging applications*—capture and analyze visible, chemiluminescent or fluorescent stained colonies on single- or multi-well cell culture plates using the optimized imaging channels and onboard analysis workflows
Flexible connectivity—export captured images via ethernet connection, Wi-Fi (with optional accessory), USB, or directly to our Connect cloud-based platform
Multiple image analysis options—perform densitometry, quantitation, and normalization directly using the on-instrument software, or for more in-depth analysis use iBright Analysis Software (available in both desktop and cloud-based versions)

*Certain functions are made available through firmware. Ensure your system is running the most up to date firmware version.

Download the latest firmware and analysis software ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
IncludesImaging Sample Blot, Preinstalled filter sets, Safe Imager Viewing Glasses, White Screen for stained SDS-PAGE gel analysis, iBright Imager Quick Start Guide
Resolution9.1 MP
Dimensions68 x 38 x 60 cm
FormatImage formats: G2i (proprietary), TIFF, JPG, PNG, PDF
Weight50 kg (110 lb.)
Field22.5 cm x 18.0 cm (WxD)
Shipping ConditionRoom Temperature
For Use With (Application)Chemiluminescent Western Blot Imaging, DNA Gel Imaging, Protein Gel Imaging
Product LineiBright
Product TypeImaging System
Unit SizeEach

Frequently asked questions (FAQs)

Do the iBright imagers support total protein normalization?

iBright Imaging Systems support total lane protein-based normalization and housekeeping gene (HKP)-based normalization. To support the methods best suited for your experiment, iBright Imaging Systems and iBright Analysis Software provide multiple quantitation and normalization options to monitor or mathematically compensate for experimental or sample variability.

To learn more about total lane protein normalization, explore our application note.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Are the iBright Imaging Systems network-compatible?

The iBright Imaging Systems support Ethernet network connections. WIFI is also supported with the addition of a USB-WIFI dongle. Data may be exported from the instrument via network and WIFI connections. It can also be exported via USB port.

For additional information on iBright functionality, visit this page.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What fluorophores can I image with the iBright FL1500 Imaging System?

The iBright FL1500 Imaging System supports a wide range of dyes, stains and fluorophores in the visible and NIR wavelength range.

For more information on compatible reagents and applications, visit this page.

To learn more about the iBright system-supported fluorophores and installed filters and light sources, visit Fluorescence SpectraViewer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is a computer needed to control the iBright Imaging Systems?

iBright Imaging Systems feature a built-in computer interface controlled by the 12.1 inch touchscreen. No additional computer is needed to operate the instrument.

For additional information on iBright functionality, visit this page.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why do the iBright Imaging Systems use green LED illumination for DNA imaging?

The iBright Imaging Systems utilize a transilluminator based on green LEDs, which effectively excite popular DNA dyes such ethidium bromide and SYBR Green dye and offer several additional benefits:

- No harmful UV rays: While UV light effectively excites many fluorescent dyes and stains, UV light is a health hazard. Further, prolonged exposure to UV light can damage DNA samples, and compromise the integrity of samples to be used for downstream applications, such as subcloning.
- No mercury waste: UV transilluminator bulbs may contain mercury, a hazardous substance, and therefore require special care for handling and disposal.
- Longer lifetime: LED bulbs have a substantially longer real-time life than fluorescent UV bulbs, which can add up to considerable cost savings over the lifetime of the instrument.

For additional information on iBright functionality, visit this page.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.