SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS
SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS
Thermo Scientific™

SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS

This substrate bundle contains SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Pico PLUSRead more
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Catalog number A45917
Price (USD)
485.00
Each
Add to cart
Price (USD)
485.00
Each
Add to cart
This substrate bundle contains SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate
Thermo Scientific SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is designed for detection of very low-abundant targets or the maximum levels of sensitivity required when using precious samples or costly antibodies.

• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

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SuperSignal West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis. It is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for routine western blotting applications.

• Sensitivity: picogram to femtogram
• Stability: eight hours for working solution; one year for kit at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

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For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeSubstrate
Detection MethodChemiluminescence
FormLiquid
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LineSuperSignal™
Quantity1 Bundle
SubstrateHorseradish Peroxidase
SensitivityPicogram to femtogram, low-femtogram to high-attogram
Sufficient For12 Mini-Gel Blots, 1000 sq. cm of Membrane; 2 Mini-Gel Blots, 200 sq. cm of Membrane
Shipping ConditionApproved for shipment at Room Temperature or on Wet or Dry Ice
Unit SizeEach
Contents & Storage
• 100 mL SuperSignal West Atto reagent, store at 2–8°C
• 20 mL SuperSignal West Pico PLUS reagent, store at room temperature

Frequently asked questions (FAQs)

How do I handle saturated or burned out bands using SuperSignal West Atto Ultimate Sensitivity Substrate?

Here are some suggestions:
- Shorten the time of exposure
- Wash blot and try using SuperSignal West Pico PLUS Chemiluminescent Substrate
- Strip the membrane using Restore Western Blot Stripping Buffer, re-probe with lesser amount of primary and secondary antibodies, and try first with SuperSignal West Pico PLUS Chemiluminescent Substrate
- Re-run the gel using lesser sample

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How can I reduce background with SuperSignal West Atto Ultimate Sensitivity Substrate?

Here are potential causes for high background and corresponding solutions:
- Too much HRP in the system: Further dilute the HRP-conjugate, see guidelines in Table 1 in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018858_SuperSignalWestAtto_UG.pdf).
- Inadequate blocking: Optimize blocking conditions.
- Inappropriate blocking reagent: Try a different blocking reagent. Use clear milk or StartingBlock blocking buffer instead of dry milk.
- Inadequate washing: Increase length, number or volume of washes.
- Film has been overexposed: Decrease exposure time or use Pierce Background Eliminator (Cat No. 21065).
- Concentration of antigen or antibody is too high: Decrease amount of antigen or antibody.
- Poor antibody specificity: Use SuperSignal Western Blot Enhancer (Cat. No. 46640).
We also recommend minimizing handling of the membrane, using clean gloves while handling, clean plasticware, glassware, forceps, or other contact surfaces. We also recommend trimming of the membrane to remove excess area.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is SuperSignal West Atto Ultimate Sensitivity Substrate compatible with Restore Western Blot Stripping Buffer?

SuperSignal West Atto Ultimate Sensitivity Substrate is compatible with Restore Western Blot Stripping Buffer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When should I use SuperSignal West Atto Ultimate Sensitivity Substrate?

We recommend using SuperSignal West Atto Ultimate Sensitivity Substrate for detection of proteins in the following situations:
- For proteins with low concentration, not measurable by BCA or Bradford assays
- For low-abundance phosphorylated or glycosylated proteins
- For low-abundance membrane or nuclear proteins
- For low-abundance proteins being detected with a low-affinity antibody or when the antibody is precious
- When sample source is limited or when multiple conditions or assays are being tested on a single sample

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is SuperSignal West Atto Ultimate Sensitivity Substrate affected by freeze/thaw cycles?

SuperSignal West Atto Ultimate Sensitivity Substrate can tolerate up to 3 freeze/thaw cycles without significant loss in performance.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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