CTS™ Viral Production Cells
CTS™ Viral Production Cells
Gibco™

CTS™ Viral Production Cells

CTS Viral Production Cells are derived from the human HEK293 cell line and are a core component of the CTSRead more
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Catalog number A53956
Price (USD)
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CTS Viral Production Cells are derived from the human HEK293 cell line and are a core component of the CTS LV-MAX lentiviral production system to produce maximal lentiviral titers. The cells allow high density, serum-free suspension culture with minimal cell clumping in CTS LV-MAX Production Medium. They are specifically validated for lentiviral vector production for the purpose of cell and gene therapy applications, but may be suitable for the production of other viral vectors where the absence of SV40 large T antigen in HEK293 cells is desired. These cGMP-banked cells have gone through a documented validation process to ensure that they can be transfected with multiple plasmids and that viable viral particles can be produced.

Features of the CTS Viral Production Cells include:

  • Fully-documented cGMP-banked cell line (HEK293F-derived) with detailed cell line lineage history
  • Extensive quality testing based on the US FDA and European Medicines Agency (EMA) guidances for therapeutic use
  • Flexible licensing options without royalties

Please visit www.thermofisher.com/celllicenserequest for more information and access.

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.
Specifications
Cell Line293F
Cell TypeKidney (Embryonic)
Culture TypeSuspension Cell Culture
FormatCryopreserved vial
For Use With (Application)Cell & Gene Therapy Research, Development, and Manufacturing
Media RecommendationGibco™ CTS™ Viral Production Medium
No. of CellsSee Certificate of Analysis
Product TypeViral Production Cell Line
Quantity1.5 mL
Shipping ConditionDry ice or vapor phase liquid nitrogen
Tested ForLV Production
Manufacturing Quality21 CFR Parts 210 and 211
Shelf Life10 years from date of manufacture
Unit SizeEach
Contents & Storage
1 x 107 cells in a 2-mL vial. Store in vapor phase liquid nitrogen.

Frequently asked questions (FAQs)

Are CTS Viral Production Cells 293T-derived?

No, CTS Viral Production Cells are HEK 293F suspension cells that have been adapted to CTS LV-MAX Production Medium. They do not express the SV40 large T antigen.

How do I bank CTS Viral Production Cells?

CTS Viral Production Cells can be frozen directly in cryogenic medium consisting of 90% CTS LV-MAX Production Medium plus 10% DMSO. Here is the protocol:

- Bank cells at passage 3 when cell density reaches ˜3.0-4.5 x 10E6 viable cells/mL at >95% viability.
- Centrifuge the cells at 100 x g (low speed) for 5 mins to pellet. Discard the spent medium.
- Prepare freezing medium: e.g., 10 mL of freezing medium is 9 mL of CTS LV-MAX Production Medium + 1 mL DMSO; mix well and sterilize by 0.2 µm filtration.
- Resuspend the cell pellet in the prepared freezing medium.
- Dilute the cells to a final density of 10 x10E6 viable cells/mL and aliquot 1 mL per cryovial.
- Freeze the cells at -80 degrees C for 1 day.
- Transfer frozen vials to liquid nitrogen for long-term storage.

Can I use CTS Viral Production Cells (Cat. No. A53956) with CTS AAV MAX products?

CTS Viral Production Cells 2.0 are specifically designed to work with CTS AAV MAX products to provide maximal adeno-associated virus (AAV) titers and optimal performance. CTS Viral Production Cells can be used but we don't recommend doing this as it will reduce system performance.