TrueMark™ STI Plus Combo Kit
TrueMark™ STI Plus Combo Kit
TrueMark™ STI Plus Combo Kit
TrueMark™ STI Plus Combo Kit
Applied Biosystems™

TrueMark™ STI Plus Combo Kit

The Applied Biosystems TrueMark STI Plus Combo Kit achieves molecular detection through real-time PCR technology, offering an end-to-end workflow forRead more
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Catalog number A56291C
Price (USD)
1,578.00
Each
Add to cart
Price (USD)
1,578.00
Each
Add to cart
The Applied Biosystems TrueMark STI Plus Combo Kit achieves molecular detection through real-time PCR technology, offering an end-to-end workflow for many common infectious disease areas by utilizing the power of QuantStudio real-time PCR systems. The combination of a TrueMark panel (included) and a QuantStudio system helps enable rapid, accurate detection and categorization of microorganisms that cause STI diseases.

The TrueMarkSTI Plus panel is composed of convenient, duplexed TaqMan assays that detect Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, Ureaplasma urealyticum, HSV1/HSV2, and an internal control in the same reaction, utilizing real-time PCR techniques.

Features of the TrueMark STI Plus panel include:
Ease-of-use—convenient, duplexed reactions include assay and positive control, pre-spotted and dried down on TaqMan plates, designed for easy setup and increased accuracy; just add sample and TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX) (included)
Endogenous controls—duplex reactions offer RNase P human internal control in every well for added confidence
Turn-around time—choose from a selection of predefined panels with a short turn-around time
Amplification control—TrueMark Amplification Control I covers all targets in RTI, UTI, STI, and vaginal panels
Maximize the workflow—expand from SARS-CoV-2 testing to TrueMark infectious disease research panels utilizing the same workflow

The TrueMark STI Plus Combo Kit includes the following components:
TrueMark STI Plus Panel—collection of duplex assays that contain primer/probe sets specific to Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, Ureaplasma urealyticum, HSV1/HSV2, and RNase P genomic regions
TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX)—ready-to-use PCR mix, including reverse transcriptase, polymerase, dNTPs, salt, and buffer

Product use
The TrueMark STI Plus Panel is a collection of duplex assays that detect microbes and an internal control in the same reaction for qualitative detection and identification of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, Ureaplasma urealyticum, HSV1/HSV2 in vaginal swabs obtained from individuals exhibiting signs and symptoms of sexually transmitted infection that cause cervicitis/inflammation of the cervix.

Specifications
Detection MethodPrimer-probe
Label or Dye2 Color
Quantity5 Plates
TargetChlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, Ureaplasma urealyticum, and HSV1/HSV2
For Use With (Application)Pathogen Detection
CompatibilityQuantStudio™ 5 Real‐Time PCR System, 96-well, 0.2-mL block; QuantStudio™ 5 Dx Real‐Time PCR System, 96-well, 0.2-mL block; QuantStudio™ 7 Pro Real-Time PCR System, 96-well, 0.2-mL block
No. of ReactionsUp to 12 Samples/Plate
Unit SizeEach
Contents & Storage
5 plates; 3 x 1 mL
Store panels at 15–30°C.
Store master mix at 5 to -30°C.

Frequently asked questions (FAQs)

Does Thermo Fisher Scientific offer analytical validation (AV) consulting services?

When adopting a new testing method for your research needs, we can assist in enabling the verification of the workflow to help ensure a successful launch. Our analytical validation (AV) consulting services can speed up the launch process by up to 75% and cut costs by up to half. Our expert team will consult to help develop and optimize your research workflow, while providing control material, data analysis support, and template documentation to fully maximize your instrument and reagent investment. Please refer to https://www.thermofisher.com/av or contact your sales representative for more information.

What specimen types can be used with the TrueMark Infectious Disease panels?

The recommended specimens that can be used with the TrueMark Infectious Disease panels are listed below:
- TrueMark Respiratory I Plus Combo Kit, TrueMark Respiratory II Plus Combo Kit, and TrueMark Respiratory III Plus Combo Kit: Nasopharyngeal (NP) or nasopharyngeal aspirate swabs
- TrueMark Urinary I Plus Combo Kit, TrueMark Urinary II Plus Combo Kit, and TrueMark Urinary III Plus Combo Kit: Urine samples
- TrueMark STI Plus Combo Kit: Vaginal and genital swabs
- TrueMark Vaginal Plus Combo Kit: Vaginal swabs
- TrueMark Lesion Plus Combo Kit: Lesion/genital swab
- TrueMark Genital Plus Combo Kit: Genital swab

What are the differences between the controls listed in the TrueMark Infectious Disease panels user guide?

Here are the controls listed in the TrueMark Infectious Disease panels user guide and their considerations for use:

TrueMark Amplification Control (Cat. No. A55698) or TrueMark Amplification Control I, low conc. (Cat. No. A55699):
- Added to a single well per target in the real-time PCR plate as a positive control. The recommended concentration for the positive control is 10,000 copies/reaction.
- Monitors real-time PCR reaction setup and reagent integrity.
- Can be ordered separately.
- Includes all the targets in the TrueMark panels.

Negative (extraction) Control:
- It is a negative control on the extraction sample plate that contains water.
- The negative (extraction) control is run on the real-time PCR plate.
- Monitors cross-contamination of reagents during reaction setup.

TaqMan Universal Extraction Control Organism (B. atrophaeus) (Cat. No. A39180):
- Used only for TrueMark Urinary plates.
- Contains three vials of lyophilized Bacillus atrophaeus, for use as a process control for cell lysis and nucleic acid recovery.
- Like other gram-positive bacteria, Bacillus atrophaeus has thick cell walls that can be difficult to lyse. This characteristic makes Bacillus atrophaeus an ideal control to monitor the efficiency of cell lysis and subsequent nucleic acid recovery.
- Is supplied lyophilized with a quantity of 1 x 10E9 copies/vial and is reconstituted in 200 µL of PBS (1X), pH 7.4 to a final concentration of 5 x 10E6 copies/µL.
- The following B. atrophaeus dilution and spike-in process instructions are recommended:
Vortex for 30 seconds, until the pellet is resuspended. Make the suitable number of aliquots and stored at -20 degrees C for up to 4 months. Perform further dilution to obtain the working concentration of 5 x 10E4 copies/µL. 10 µL of the control is added to urine samples in each extraction well at the start of extraction.

What is the approximate workflow time for running the TrueMark Infectious Disease panel assay starting from sample preparation?

It takes approximately 3 hours for running the assay starting from sample preparation to receiving the Ct value.

With TrueMark Infectious Disease panels, how long can we hold the samples before starting the procedure?

We recommend running the plate immediately. If the plate is not ready to run, store it at 2-8 degrees C for up to 2 hours.