Fetal Bovine Serum, Premium Plus
Fetal Bovine Serum, Premium Plus
Fetal Bovine Serum, Premium Plus
Fetal Bovine Serum, Premium Plus
Fetal Bovine Serum, Premium Plus
Gibco™

Fetal Bovine Serum, Premium Plus

Green features
Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust ofRead more
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Catalog NumberQuantityFormat
A5669701500 mLBottle
A566940150 mLOne Shot
A566940210 x 50 mLOne Shot
Catalog number A5669701
Price (USD)
924.00
Each
Add to cart
Quantity:
500 mL
Format:
Bottle
Price (USD)
924.00
Each
Add to cart

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements.

Sera category: Premium Plus

  • Use for a broad range of cell types, especially sensitive cell lines
  • Our most characterized serum with the lowest endotoxin and hemoglobin levels
  • Endotoxin level: ≤5 EU/mL
  • Hemoglobin level: ≤20 mg/dL
  • Origin: Refer to CoA for specific origin (US, Australia, New Zealand)
  • US Department of Agriculture (USDA) safety tested for the absence of bluetongue and akabane from countries that are USDA-approved for importation into the US

Gibco Serum Delivers

  • ISO 13485-certified; processed in FDA-registered facilities
  • Triple filtered at 0.1 μm
  • Gibco bottle easier to use in hood, reduces risk of contamination and helps improve consistency
  • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AgeFetal
SpeciesCattle/Bovine
Country of OriginRefer to CoA for specific origin
FormFrozen
FormatBottle
Product TypeFetal Bovine Serum
Quantity500 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Sterilization MethodTriple-filtered, 0.1 μm
GradePremium Plus
ProcessingTriple-filtered, 0.1μm
Tested ForUp to 70 quality tests including EMA virus testing; USP/EP mycoplasma, endotoxin, performance; biochemical/hormonal profiling; Oritain fingerprinting
Endotoxin Concentration≤5 EU/mL
Green FeaturesLess waste
Hemoglobin Concentration≤20 mg/dL
Shelf Life5 Years
Shipping ConditionDry Ice
USDA Safety Testing IncludedUSDA Safety Testing Performed for Australian origins
Unit SizeEach
Contents & Storage
Store at ≤-10°C.

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, Premium Plus FAQs