Fetal Bovine Serum (FBS), Premium, heat-inactivated
Fetal Bovine Serum (FBS), Premium, heat-inactivated
Fetal Bovine Serum (FBS), Premium, heat-inactivated
Fetal Bovine Serum (FBS), Premium, heat-inactivated
Fetal Bovine Serum (FBS), Premium, heat-inactivated
Gibco™

Fetal Bovine Serum (FBS), Premium, heat-inactivated

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust ofRead more
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Catalog NumberQuantityFormat
A5670801500 mLBottle
A567050150 mLOne Shot
A567050210 x 50 mLOne Shot
Catalog number A5670801
Price (USD)
828.00
Each
Add to cart
Quantity:
500 mL
Format:
Bottle
Price (USD)
828.00
Each
Add to cart

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements.

Gibco sera: trusted quality for consistent performance

Sera category: Premium

  • Use for general cell culture with common cell lines
  • Low endotoxin and hemoglobin levels
  • Endotoxin level: <10 EU/mL
  • Hemoglobin level: <25 mg/dL
  • Origin: refer to CoA for specific origin. All products may not be available in all regions due to importation regulations.
  • US Department of Agriculture (USDA) safety tested for the absence of bluetongue and akabane from countries that are USDA-approved for importation into the US

Gibco serum delivers

  • ISO 13485–certified; processed in FDA registered facilities
  • Triple-filtered at 0.1 μm
  • Gibco bottle easier to use in hood; helps reduce risk of contamination and improve consistency
  • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook
Specifications
AgeFetal
SpeciesCattle/Bovine
Country of OriginRefer to CoA for specific origin
FormFrozen
FormatBottle
Product TypeFetal Bovine Serum
Quantity500 mL
Serum TreatmentHeat-inactivated
SterilitySterile-filtered
Sterilization MethodTriple-filtered, 0.1 μm
GradePremium
Tested ForUp to 60 quality tests including 9CFR virus testing, endotoxin, and performance
Endotoxin Concentration≤10 EU/mL
Green FeaturesLess waste
Hemoglobin Concentration≤25 mg/dL
Purity or Quality GradePremium
Shelf Life5 Years
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Store at ≤-10°C.

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum (FBS), Premium, heat-inactivated FAQs