Verso cDNA Synthesis Kit
Verso cDNA Synthesis Kit
Thermo Scientific™

Verso cDNA Synthesis Kit

The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool. The VersoRead more
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Catalog NumberNo. of Reactions
AB1453B100 Reactions
AB1453A40 Reactions
Catalog number AB1453B
Price (USD)
528.00
Each
Add to cart
No. of Reactions:
100 Reactions
Price (USD)
528.00
Each
Add to cart

The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool. The Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.

Features of the Verso cDNA Synthesis Kit include:
• Highly sensitive with a broad dynamic range—can reverse transcribe RNA from an input range of 1pg to 1μg
• Efficient RT reaction allows for 75% shorter protocol times (down to 30 minutes)
• Unique priming strategy for increased efficiency and broad template flexibility
• High affinity RT enzyme effectively transcribes through difficult RNA sequences
• Wide working temperature range (42–57°C) for success with GC-rich and other difficult templates
• Included RT enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment
• RNase inhibitor included
• Can be paired with any PCR Kit for complete 2-Step RT-(q)PCR

Applications
• 2-step RT-(q)PCR
• Single stranded cDNA synthesis

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Reverse TranscriptaseVerso
Ribonuclease H ActivityYes
Shipping ConditionWet or Dry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Final Product TypeFirst-Strand cDNA
No. of Reactions100 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 11 kb
Starting MaterialRNA
Unit SizeEach
Contents & Storage

• RT Enzyme mix with RNase Inhibitor
• 5X RT Buffer
• Random Hexamers
• Anchored Oligo (dT)
• 20 mM dNTPs
• RT Enhancer

Store at –20°C.

Frequently asked questions (FAQs)

What is the RT Enhancer that is included in all Verso kits?

The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.

Do I need to treat my RNA with DNase I before running RT-qPCR with Verso cDNA Synthesis Kit?

No, the Verso cDNA Syntheis Kits include RT Enhancer that, when included in the cDNA synthesis reaction, removes contaminating DNA eliminating the need for DNase I treatment.

What is the error rate of the Verso reverse transcription enzyme (Cat. No. AB1453A, AB1453B)?

Error rate is approximately 1 error per 15-27x 10^3 bases.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What is the size of cDNA that can be obtained from the Verso cDNA Synthesis Kit (Cat. No. AB1453A, AB1453B)

The Verso can synthesize long cDNA strands, up to 11 kb, at a temperature range of 42°C to 57°C.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What are the features of the Verso cDNA Synthesis Kit (Cat. No. AB1453A, AB1453B)?

The Verso cDNA synthesis Kit enables robust transcription of RNA to create a complete cDNA pool. The Verso system allows robust and sensitive reverse transcription through the combination of a high-affinity RT enzyme, a trusted RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.