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AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverseRead more
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| Catalog Number | No. of Reactions |
|---|---|
| AM1005 | 100 Reactions |
| 4387424 | 500 Reactions |
| 4387391 | 1000 Reactions |
Catalog number AM1005
Price (USD)
263.00
Each
No. of Reactions:
100 Reactions
Price (USD)
263.00
Each
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalization
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalization
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Veterinary Use Only.
Specifications
DescriptionWorks on all AB platforms, but is optimized for the 7500.
Detection MethodPrimer-probe
FormatTube
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-qPCR
PolymeraseAmpliTaq Gold DNA Polymerase
Reaction SpeedStandard
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Passive Reference DyeROX (Pre-mixed)
Product LineAgPath-ID™, Ambion™
Product TypeOne-Step RT-PCR Reagent
Reverse TranscriptaseArrayScript™
Shipping ConditionDry Ice
Sufficient For100 Reactions
Concentration25X
Fidelity (vs. Taq)25 X
No. of Reactions100 Reactions
Sample TypeRNA
Volume1.75 mL
Unit SizeEach
Contents & Storage
Sufficient for 100 reactions of 25 μL, contains:
• 1375μL 2X RT-PCR Buffer
• 110μL 25X RT-PCR Enzyme Mix
• 1.75mL Nuclease-free Water.
• 1375μL 2X RT-PCR Buffer
• 110μL 25X RT-PCR Enzyme Mix
• 1.75mL Nuclease-free Water.