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MEGAscript™ SP6 Transcription Kit
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA producedRead more
| Catalog Number | Product Type | Quantity |
|---|---|---|
| AM1330 | SP6 In Vitro Transcription kit | 40 Reactions |
Catalog number AM1330
Price (USD)
433.00
Each
Product Type:
SP6 In Vitro Transcription kit
Quantity:
40 Reactions
Price (USD)
433.00
Each
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion™ kit includes sufficient reagents for 40 reactions.
• Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
• Amplifies aRNA for gene array analysis and other applications
• Efficiently incorporates many modified nucleotides
A typical 20 μl MEGAscript™ reaction with 1 μg of the pTRI-Xef-1alpha control template will yield over 100 μg of transcript. The versatility of the MEGAscript™ kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase.
Accessory Products:
MessageAmp™ aRNA Amplification Kits are powered by MEGAscript™ technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, Ambion recommends using the MEGAclear™ Kit (SKU#AM1908) and the MEGAclear™-96 Kit (SKU#AM1909).
• Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
• Amplifies aRNA for gene array analysis and other applications
• Efficiently incorporates many modified nucleotides
A typical 20 μl MEGAscript™ reaction with 1 μg of the pTRI-Xef-1alpha control template will yield over 100 μg of transcript. The versatility of the MEGAscript™ kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase.
Accessory Products:
MessageAmp™ aRNA Amplification Kits are powered by MEGAscript™ technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, Ambion recommends using the MEGAclear™ Kit (SKU#AM1908) and the MEGAclear™-96 Kit (SKU#AM1909).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeSynthetic mRNA, mRNA
FormatKit
Sample TypemRNA, Synthetic mRNA
For Use With (Application)In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions40 Reactions
Product LineAmbion™, MEGAscript™
Product TypeSP6 In Vitro Transcription kit
Quantity40 Reactions
PromoterSP6
Shipping ConditionDry Ice
Yield100 μg
Starting MaterialPCR Products, Linearized DNA plasmid
Unit SizeEach
Contents & Storage
SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.