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Invitrogen™
RNAqueous™ Total RNA Isolation Kit
The RNAqueous™ Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using aRead more
Catalog number AM1912
Price (USD)
438.00
Each
Price (USD)
438.00
Each
The RNAqueous™ Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous™ Kit provides:
• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous™ Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous™ Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologySpin Column (Glass Fiber Filter)
Elution Volume50–120 μL
Sample TypeBacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
Final Product TypeTotal RNA
High-throughput CompatibilityNot High-throughput Compatible (Manual)
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity50 Preps
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤109 cells
Cells: ≤107
Tissue: ≤75 mg
Plant: ≤75 mg
Yeast: ≤108 cells
Virus: varies
Enzymatic Reactions: varies
Cells: ≤107
Tissue: ≤75 mg
Plant: ≤75 mg
Yeast: ≤108 cells
Virus: varies
Enzymatic Reactions: varies
Unit SizeEach
Contents & Storage
• 6 mL Formaldehyde Load Dye; store at -20°C
• 60 mL Lysis/Binding Solution; 4°C
• 60 mL Water; 4°C
• 4 mL Lithium Chloride Precipitation Solution; 4°C
• 40 mL Wash Solution #1; 4°C
• 80 mL Wash Solution #2/3 (concentrated); 4°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes; room temperature
• 10 mL Elution Solution; room temperature
• 60 mL Lysis/Binding Solution; 4°C
• 60 mL Water; 4°C
• 4 mL Lithium Chloride Precipitation Solution; 4°C
• 40 mL Wash Solution #1; 4°C
• 80 mL Wash Solution #2/3 (concentrated); 4°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes; room temperature
• 10 mL Elution Solution; room temperature