Salmon Sperm DNA, sheared (10 mg/mL)

Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100–200 μg/mL.

Using this product
This sheared salmon sperm DNA has been treated with Proteinase K to remove any contaminating nucleases, followed by organic extraction with phenol:chloroform and ethanol precipitation. Salmon Sperm DNA is rigorously tested for RNase and DNase contamination and is suspended in nuclease-free water in a 1 mL tube at a concentration of 10 mg/mL (1 mL per tube).