BacLight™ RedoxSensor™ Green Vitality Kit
<i>Bac</i>Light&trade; RedoxSensor&trade; Green Vitality Kit
Invitrogen™

BacLight™ RedoxSensor™ Green Vitality Kit

The RedoxSensor™ Green reagent included in the BacLight™ RedoxSensor™ Green Vitality Kit is an indicator of bacterial reductase activity. ThisRead more
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Catalog number B34954
Price (USD)
612.00
Each
Add to cart
Price (USD)
612.00
Each
Add to cart
The RedoxSensor™ Green reagent included in the BacLight™ RedoxSensor™ Green Vitality Kit is an indicator of bacterial reductase activity. This reductase activity is, in turn, a reliable marker for changes in electron transport chain function and for changes in vitality that occurs following antibiotic treatment. RedoxSensor™ Green reagent penetrates both gram-positive and gram-negative bacteria. Following reduction, the RedoxSensor™ Green reagent will produce a stable green-fluorescent signal in 10 minutes that is compatible with formaldehyde fixation techniques.

View additional information about all microbiology assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeBacteria
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product TypeGreen Vitality Kit
Dye TypeRedoxSensor™ green, PI
EmissionPI: 490⁄635, RedoxSensor™ green: 490⁄520
FormSolution
FormatTube(s), Slide(s)
Product LineBacLight™, RedoxSensor™
Quantity200
Shipping ConditionRoom Temperature
SolubilityDMSO (Dimethylsulfoxide)
Unit SizeEach
Contents & Storage
Contains 1 vial of RedoxSensor™ green (200 μL, 1 mM solution in DMSO), 1 vial of propidium iodide (300 μL, 20 mM solution in DMSO), 1 vial of sodium azide (1 mL of a 2 M solution in water), and 1 vial of CCCP (400 μL, 5mM in DMSO).

Frequently asked questions (FAQs)

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the excitation/emission of the RedoxSensor Green reagent?

The excitation/emission of the RedoxSensor Green reagent, in reduced form, is 490/520 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.