DiSBAC2(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)
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DiSBAC<sub>2</sub>(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)
Invitrogen™

DiSBAC2(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)

The slow-response potential-sensitive probe, DiSBAC2(3) can enter depolarized cells where it binds to intracellular proteins or membrane and exhibits enhancedRead more
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Catalog number B413
Price (USD)
331.00
Each
Add to cart
Price (USD)
331.00
Each
Add to cart
The slow-response potential-sensitive probe, DiSBAC2(3) can enter depolarized cells where it binds to intracellular proteins or membrane and exhibits enhanced fluorescence and a red spectral shift. Increased depolarization results in additional influx of the anionic dye and an increase in fluorescence. Conversely, hyperpolarization is indicated by a decrease in fluorescence. Potential-dependent fluorescence changes generated by this bis-oxonol are typically ∼1% per mV. Also, this probe has an excitation maxima of 530 nm and emission maxima of 560 nm. DiBAC dyes are excluded from mitochondria because of their overall negative charge, making them superior to carbocyanines for measuring plasma membrane potentials.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeDiSBAC2(3)
Dye TypeMembrane Potential Probes
Quantity100 mg
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.