Bolt™ Transfer Buffer (20X)
Bolt™ Transfer Buffer (20X)
Invitrogen™

Bolt™ Transfer Buffer (20X)

Bolt Transfer Buffer (20X) is used to transfer proteins from Bolt Bis-Tris Plus gels to membranes for western blotting. ItRead more
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Catalog NumberQuantity
BT0006125mL
BT000611L
Catalog number BT0006
Price (USD)
33.55
Each
Add to cart
Quantity:
125mL
Price (USD)
33.55
Each
Add to cart
Bolt Transfer Buffer (20X) is used to transfer proteins from Bolt Bis-Tris Plus gels to membranes for western blotting. It maintains the neutral pH environment established during electrophoresis. The neutral pH protects against modification of amino acid side chains and is compatible with N-terminal protein sequencing using Edman degradation.

Bolt Antioxidant may be used with Bolt Transfer Buffer to enhance transfer of reduced proteins to membranes.

See all available buffers and reagents available for SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferTransfer Buffers
Concentration20 X
Product TypeWestern Blot Transfer Buffer
Volume (Metric)125 ml
FormLiquid
Gel CompatibilityBolt™ Bis-Tris Plus Gels
Product LineBolt™
Quantity125mL
Unit SizeEach
Contents & Storage
Store at 4 to 25°C.

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

View all Bolt™ Transfer Buffer (20X) FAQs