Human Epidermal Keratinocytes, neonatal (HEKn)
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Human Epidermal Keratinocytes, neonatal (HEKn)

Primary Normal Human Epidermal Keratinocytes, neonatal (HEKn) are isolated from neonatal foreskin and cryopreserved at the end of the primaryRead more
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Catalog number C0015C
Price (USD)
696.00
Each
Add to cart
Price (USD)
696.00
Each
Add to cart
Primary Normal Human Epidermal Keratinocytes, neonatal (HEKn) are isolated from neonatal foreskin and cryopreserved at the end of the primary culture.

The cells are:
• Performance tested—at least 30 population doublings when cultured in EpiLife Medium supplemented with HKGS (for HEKn) or Supplement S7 (for HEKn-AOF)
• Guaranteed to be ≥ 70% viable
• Tested for mycoplasma, bacteria, yeast or other fungi, hepatitis B, hepatitis C, and HIV-1 viruses

Each vial contains over 0.5 x106 viable cells that have been cryopreserved at the end of the primary culture stage in a medium containing 10% DMSO.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AgeNewborn (14 days or less)
Cell TypeKeratinocyte Cells
Culture TypeAdherent Cell Culture
FormCryopreserved
SpeciesHuman
Donor SourceSingle Donor
No. of Cells≥500000
Product LineCascade Biologics™
Product TypeHuman Epidermal Keratinocyte
Quantity1 vial
Tested ForHepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria & Fungi), Mycoplasma, Growth Performance Assay, HIV-1
Unit SizeEach
Contents & Storage
Store in liquid nitrogen.

Frequently asked questions (FAQs)

I see large, flat pancake-looking cells in my keratinocyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care an attention, the culture should yield at least 25 population doublings.

I see large, flat pancake-looking cells in my Human Keratinodyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size, should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care and attention, the culture should yield at least 25 population doublings.

What is the doubling time for Human Keratinodyte?

Each lot is tested for doubling time and the results are listed on the COA.

Can I specify a donor type for the primary cells you offer?

This information also appears on the COA.

What is the difference between basal cells or basal keratinocytes?

These are all the same. Sometimes Keratinocytes are referred to as basal cells or basal keratinocytes.

View all Human Epidermal Keratinocytes, neonatal (HEKn) FAQs