CyQUANT™ NF Cell Proliferation Assay
CyQUANT™ NF Cell Proliferation Assay
Invitrogen™

CyQUANT™ NF Cell Proliferation Assay

The CyQUANT™ NF Cell Proliferation Assay provides a fast and sensitive method for counting cells in a population and measuringRead more
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Catalog NumberQuantity
C350071 Kit(s)
C350061000 reactions
Catalog number C35007
Price (USD)
200.00
Each
Add to cart
Quantity:
1 Kit(s)
Price (USD)
200.00
Each
Add to cart
The CyQUANT™ NF Cell Proliferation Assay provides a fast and sensitive method for counting cells in a population and measuring proliferation in microplate format.

Features of the CyQUANT™ NF Cell Proliferation Assay:

• More sensitive than MTT or AlamarBlue™ assays
• Linear detection range from 100 to 20,000 cells per well (96-well microplate)
• Assays can be completed in one hour

A Rapid and simple method for measuring cell proliferation
The CyQUANT™ NF Cell Proliferation Assay does not require cell lysis, long incubations, radioactivity, or removal of stain from cells. The CyQUANT™ NF assay eliminates the freeze-thaw cell lysis step of the original CyQUANT™ cell proliferation assay by using a cell-permeant DNA-binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT™ NF Cell Proliferation Assay can be used in either 96-well or 384-well microplate formats, and is available in two configurations: a 200 assay kit (C35007) for smaller sample sizes, and a 1000 assay kit (C35006) for high-throughput applications.

AlamarBlue™ is a registered trademark of TREK Diagnostic Systems.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Application)Proliferation Assay
For Use With (Equipment)Microplate Reader
Product TypeCell Proliferation Assay
Dye TypeOther Label(s) or Dye(s)
Format384-well plate, 96-well plate
Product LineCyQUANT™
Quantity1 Kit(s)
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in refrigerator 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

What is the difference between the original CyQuant assay and the CyQuant NF assay?

The original CyQUANT assay provides sensitive detection of cells over a 1000-fold linear dynamic range. In this assay, a freeze-thaw cell lysis step is required to facilitate the interaction of the CyQUANT GR dye with DNA. The CyQUANT NF assay avoids this freeze-thaw step by using a DNA binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT NF protocol requires only aspiration of growth medium (for adherent cells), replacement with dye binding solution, incubation for 30-60 minutes, and then measurement of fluorescence in a microplate reader. The assay is designed to produce a linear analytical response from at least 100-20,000 cells per well in most cell lines in a 96-well microplate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.