MultiShot™ FlexPlate DH5α T1R Competent Cells
MultiShot&trade; FlexPlate DH5&alpha; T1<sup>R</sup> Competent Cells
Invitrogen™

MultiShot™ FlexPlate DH5α T1R Competent Cells

MultiShot FlexPlate DH5α T1R Chemically Competent E. coli cells are cloning competent cells derived from a well-known, versatile DH5α strainRead more
Have Questions?
Catalog number C4481201
Price (USD)
608.00
Each
Add to cart
Price (USD)
608.00
Each
Add to cart

MultiShot FlexPlate DH5α T1R Chemically Competent E. coli cells are cloning competent cells derived from a well-known, versatile DH5α strain that can be used in many everyday cloning applications. These cells are pre-aliquoted in a 96-well PCR plate to increase transformation productivity, and the FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

All of our T1R strains have the tonA (also known as fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries.

Reliable DH5α T1R cloning capabilities
MultiShot FlexPlate DH5α T1R competent E. coli cells are able to achieve transformation efficiencies of >1 x 108 cfu/μg control DNA. This strain is recommended for transformations of cloned unmethylated DNA, routine cloning of genes into large plasmids, and is suitable for difficult clone constructions, such as blunt-end ligations or cloning using limited amount of DNA.

The DH5α T1R strain carries the hsdR mutation to prevent restriction of foreign DNA and the endA1 mutation that increases the yield of plasmid DNA prepared using miniprep protocols. The lacZΔM15 marker provides α-complementation of the ß-galactosidase gene allowing blue-white screening on agar plates containing X-Gal or Bluo-Gal.

MultiShot FlexPlate DH5α T1R Competent Cells offer:
• Transformation efficiency of >1 x 108 transformants/μg plasmid DNA
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• lacZΔM15 marker for blue-white screening
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Increase transformation productivity with MultiShot FlexPlate format
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key benefits of the MultiShot FlexPlate format
• Increased throughput—compatible with automated liquid handling platforms
• Flexible—transform in increments of 8 wells to entire 96-well plate
• Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype
F– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ–thi-1 gyrA96 relA1 tonA

Find the strain and format that fits your needs
Many other E. coli strains and formats of chemically competent and electrocompetent cells are also available to meet specific transformation needs. For high-throughput transformation, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options. We also offer bacterial growth media in various formats.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeChemically Competent Cells
Contains F' EpisomeNo
Cloning Methylated DNANo
Antibiotic Resistance BacterialNo
Blue/White ScreeningYes (lacZΔM15)
High-throughput CompatibilityHigh
Preparing Unmethylated DNANo
Reduces RecombinationYes (recA1)
Shipping ConditionDry Ice
T1 Phage - Resistant (tonA)Yes
SpeciesE. coli (K12)
Format96-well Plate
Product LineMultishot™
Quantity1 x 96-well Plate
Unit SizeEach
Contents & Storage
• MultiShot FlexPlate DH5α T1R Competent Cells (1 x 96-well plate at 20 μL/well)
Store at –80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store at –20°C.

• S.O.C. Medium (2 x 10 mL)
Store at 4°C or room temperature.

Frequently asked questions (FAQs)

Is it possible to perform a fast transformation protocol with the MultiShot competent cells?

There are situations where either because of time or process limitations, elimination of the heat shock and recovery time post-heat shock in the transformation protocol would be desirable. Testing was performed on many of our chemically competent cells in both the MultiShot StripWell and MultiShot FlexPlate format with a shortened transformation protocol. Use of a rapid transformation protocol (add DNA, no heat shock, no recovery period, plate on warm agar) yielded up to a log lower in transformation efficiency using the control DNA. This rapid transformation protocol can be performed with expectation of lower colony counts than our standard high efficiency transformation protocol and can only be performed with plasmids that use ampicillin as their antibiotic resistance. The rapid protocol is ideal when using a liquid handling system for high-throughput transformations with high cloning efficiency. Please see the product manual for full details on this rapid transformation protocol option.

Can I use a thermal cycler or heat block to heat shock the competent cells in the MultiShot FlexPlate?

The MultiShot FlexPlate is a PCR plate and can be placed in a thermal cycler or heat block set at 42 degrees C to transform the cells by the heat shock method. The duration of the heat shock performed in these devices has yielded similar transformation efficiencies as that seen with heat shock done in a water bath.

Which E. coli strains do you offer in the MultiShot StripWell format?

We offer the following E. coli strains in the MultiShot StripWell format: TOP10, DH5alpha T1R, Mach1 T1R, Stbl3, and BL21 Star (DE3) chemically competent cells.

Which E. coli strains do you offer in the MultiShot FlexPlate format?

We offer the following E. coli strains in the MultiShot FlexPlate format: TOP10, DH5alpha T1R, DH10B T1R, Mach1 T1R, OmniMAX2 T1R, and Stbl3 chemically competent cells.

What are the differences between the MultiShot StripWell, MultiShot FlexPlate, and MultiShot formats of E. coli cells?

The MultiShot StripWell format is supplied as pre-aliquoted competent cells in 96 tubes contained in a rack. The tubes are attached in strips of 8 tubes, and there are 12 strips that contain 50 µL of chemically competent cells. This format offers the most flexibility to transform 1 tube or up to all 96 tubes at once and is intended for varied or medium-throughput use.

The MultiShot FlexPlate format is supplied as pre-aliquoted competent cells in a 96-well PCR plate. Each well contains 20 µL of competent cells. The plate can be separated at each column of 8 wells, offering a high level of flexibility to utilize various increments of 8 wells per transformation. The wells of the plate are heat sealed, preventing detachment of the aluminum seal in the -80 degrees C freezer and preventing contamination. This format is compatible with automated liquid handling platforms and is intended for medium- to high-throughput use.

The MultiShot format is supplied as pre-aliquoted competent cells in 5, 96-well thin-wall polycarbonate microplates. Each well of the 96-well microplate contains 15 µL of chemically competent cells. This format is compatible with automated liquid handling platforms and is intended for high-throughput use.