MultiShot™ StripWell Mach1™ T1 Phage-Resistant Chemically Competent E. coli

MultiShot StripWell Mach1 T1^R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells packaged in a rack containing 12 strips of 8 tubes for increased productivity of bacterial transformations. The Mach1 T1^R strain is derived from a non-K-12, wild-type W E. coli strain (ATCC #9637) that was selected for its rapid growth rate, and genetically manipulated to possess the most useful cloning strain features.

All of our T1^R strains have the tonA (also known as fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Accelerate cloning workflow using Mach1 T1^R strain
MultiShot StripWell Mach1 T1^R competent E. coli cells are fast growing chemically competent cells with a doubling time of approximately 50 minutes, with other popular strains averaging 70 minutes. Mach1-T1R colonies are clearly visible within 8 hours of plating the transformation mix (ampicillin selection), enabling you to plate and pick colonies the same day. You can also prepare plasmid DNA 4 hours after inoculating a single, overnight-grown colony in the selective media of choice (with any antibiotic). The Mach1 T1^R strain is useful for many cloning applications and provides transformation efficiencies in the MultiShot StripWell format of >1 x 10⁸ cfu/μg with plasmid DNA.

The Mach1 T1^R strain was engineered to carry the tonA mutation that confers resistance to T1 and T5 phage infection. The tonA genotype offers added security to valuable clones and libraries. The Mach1 T1^R strain has the lacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries the recA1398 mutation that helps reduce the rate of recombination while propagating plasmid DNA, the endA1 mutation that increases plasmid DNA yield and quantity, and hsdR restriction system deletion to allow genomic DNA cloning.

MultiShot StripWell Mach1 T1^R Competent Cells offer:
• Transformation efficiencies in StripWell format of >1 x 10⁸ cfu/μg plasmid DNA
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• lacZ∆M15 for blue-white color screening of recombinants
• hsdR mutation for efficient transformation of unmethylated DNA from PCR applications
• recA1398 mutation for reduced occurrence of homologous recombination in cloned DNA
• endA1 mutation for increased plasmid yield and quality

MultiShot StripWell flexible format
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and reducing chance of contamination. After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube.

The StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots of cells and avoiding freeze-thaws that can result in reduced transformation efficiency.

Benifits of the MultiShot StripWell format include:
• Increased productivity—suitable for high throughput experimentation
• Maximal flexibility—transform as few as 1 or as many as 96 tubes from the StripWell rack
• Familiar convenience—add DNA directly to cells, heat shock, and recover in the same tube

Genotype
F^– φ80lacZΔM15 ΔlacX74 hsdR(r_K–, m_K+) ΔrecA1398 endA1 tonA

Find the strain and format that fits your needs
A variety of E. coli strains and formats of chemically competent and electrocompetent cells are available to meet transformation needs.
For high-throughput transformation choose from our collection of MultiShot comp cells or email us for custom formatting options.
We offer bacterial growth media in various formats.