Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent
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Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent
Invitrogen™

Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent

Lipofectamine CRISPRMAX Cas9 Transfection Reagent is the first optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 protein delivery. It is aRead more
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Catalog NumberQuantity
CMAX000030.3 mL
CMAX000080.75 mL
CMAX000010.1 mL
CMAX000151.5 mL
Catalog number CMAX00003
Price (USD)
291.00
Each
Add to cart
Quantity:
0.3 mL
Price (USD)
291.00
Each
Add to cart
Lipofectamine CRISPRMAX Cas9 Transfection Reagent is the first optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 protein delivery. It is a high-throughput-friendly, cost-effective alternative to electroporation.

Working together with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA, it provides:
• Demonstrated cleavage efficiency—tested in over 20 cell types including iPSC, mESC, N2A, CHO, A549, HCT116, HeLa, HEK293, and several others
• Low cell toxicity—less cells needed to initiate your experiment
• High throughput friendly—an ideal delivery solution for 96-well format
• Cost savings—whether cost-per-reaction or initial investment

Achieve high transfection efficiency in novel genome editing applications
Lipofectamine CRISPRMAX transfection reagent increases the likelihood of successful cleavage and recombination, especially when combined with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA. This ultimately maximizes the efficiency of performing genetic modifications.

Improve experimental outcomes using TrueCut Cas9 Protein v2
Unlike CRISPR plasmid or Cas9 mRNA, using Cas9 protein provides superior cleavage efficiency in primary cells and stem cells. It eliminates the need for transcription or translation of the payload, removes the risk of genomic integration, and is cell cycle independent.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypeProtein, Ribonucleoprotein, Cas9 Ribonucleoprotein, Cas9, Plasmid DNA
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Product LineCRISPRMAX™, Lipofectamine™
Product TypeTransfection Reagent
Quantity0.3 mL
Serum CompatibleYes
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Unit SizeEach
Contents & Storage
Contains one vial of Lipofectamine CRISPRMAX Reagent and one vial of Cas9 Plus Reagent.

Store at 2–8°C. Do not freeze.

Frequently asked questions (FAQs)

Can I use Lipofectamine CRISPRMAX Cas9 Transfection Reagent with one-cell embryos?

We have no data regarding the use of CRISPRMAX in one-celled embryos, therefore we do not recommend using this product for this application.

The shipping for my Lipofectamine CRISPRMAX Cas9 Transfection Reagent was delayed. Can I still use it?

All of the Lipofectamine reagents, including Lipofectamine CRISPRMAX Cas9 Transfection Reagent, are stable at room temperature for months. What is most important is that the reagent did not freeze, because that will alter the integrity and composition of the lipid particles and will compromise usability. Previous freezing can be evidenced by precipitation and excessive cloudiness of the reagent. A limited amount of cloudiness is normal, but excessive cloudiness is a sign that the reagent froze at some point. If the reagent you received looks clear or a bit cloudy the product should still be usable. Some additional data on room temperature stability of some Lipofectamine reagents, in the context of shipping, can be found in this white paper.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How much Lipofectamine CRISPRMAX Reagent, Cas9 protein, and gRNA should I complex for gene editing?

Please see the Lipofectamine CRISPRMAX Reagent manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0014545_lipofectamine_crispermax_QR.pdf) for protocol details or contact techsupport@thermofisher.com.

How does the Lipofectamine CRISPRMAX Reagent work?

The Lipofectamine CRISPRMAX Reagent combined with the proprietary enhancement properties of the Lipofectamine Cas9 Plus Reagent leads to efficient complex formation with the Cas9-gRNA ribonucleoprotein, for best delivery to the nucleus, helping to ensure high gene editing frequency for a wide range of cell types.

Can the Lipofectamine CRISPRMAX Reagent be used to deliver proteins other than the Cas9 nuclease?

Although the Lipofectamine CRISPRMAX Reagent was developed for the delivery of the Cas9-gRNA ribonucleoprotein, there is potential for other protein complex applications.