Ethidium Monoazide Bromide (EMA)
Ethidium Monoazide Bromide (EMA)
Invitrogen™

Ethidium Monoazide Bromide (EMA)

Ethidium monoazide is a fluorescent photoafinity label that, after photolysis, binds covalently to nucleic acids both in solution and inRead more
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Catalog number E1374
Price (USD)
316.00
Each
Add to cart
Price (USD)
316.00
Each
Add to cart
Ethidium monoazide is a fluorescent photoafinity label that, after photolysis, binds covalently to nucleic acids both in solution and in cells that have compromised membranes. The fluorescence of ethidium monoazide is weak, but the intensity increases ∼15-fold on binding to DNA with excitation/emission maxima of ∼504/600 nm.

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For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorOrange
Excitation Wavelength Range504 nm
Dye TypeCell-Impermeant
For Use With (Application)Cell staining assays
For Use With (Equipment)Flow Cytometer
Quantity5 mg
Detection MethodFluorescence
DescriptionEthidium Monoazide Bromide (EMA)
Emission600 nm
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeViability Indicator
SubCellular LocalizationNucleic Acids, Nucleus
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which cell viability kits are compatible with fixation?

The LIVE/DEAD Fixable kits for flow cytometry analysis are compatible with fixation. These kits use amine-reactive cell-impermeant dyes that stain the cell surface of live cells and also the cytosol of dead cells-live cells are dim and dead cells are bright. Since the dye is covalently bound to the cells, it will be retained after fixation. Unfortunately, this method does not work well for imaging-based assays, as all cells are stained and it is difficult to distinguish bright dead cells from dim live cells with a microscope. Ethidium monoazide (EMA; Cat No. E1374) is a cell impermeant nucleic acid stain that can be applied to live cultures and stains only dead cells. After incubation and washing away unbound dye, the cells can be exposed to light to photoactivate EMA to crosslink to dead cell DNA. After crosslinking to dead cell DNA, the samples may be fixed and permeabilized. Image-IT DEAD Green Viability Stain (Cat. No. I10291) for imaging and high-content screening (HCS) analysis is a live-cell impermeant DNA binding dye that is compatible with fixation and permeabilization with good retention up to 48 hours. We also have a LIVE/DEAD Reduced Biohazard Cell Viability Kit (Cat. No. L7013) for imaging and flow analysis that contains two DNA binding dyes, SYTO 10 and Dead Red, that are sufficiently retained to be analyzed soon after 4% glutaraldehyde fixation.
Note: In general, DNA-binding dyes and calcein AM are not compatible with fixation, as these dyes are not covalently bound to components of the cell and will thus slowly diffuse out of cells after fixation, gradually staining all cells as dead.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.