ELF™ 97 Phosphatase Substrate (ELF™ 97 Phosphate), 0.2 μm Filtered
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ELF™ 97 Phosphatase Substrate (ELF™ 97 Phosphate), 0.2 μm Filtered
Invitrogen™

ELF™ 97 Phosphatase Substrate (ELF™ 97 Phosphate), 0.2 μm Filtered

The ELF™ 97 phosphatase substrate upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymaticRead more
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Catalog number E6588
Price (USD)
483.00
Each
Add to cart
Price (USD)
483.00
Each
Add to cart
The ELF™ 97 phosphatase substrate upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity. This fluorescent precipitate has several unique spectral characteristics, including an extremely large Stokes shift that makes it easily distinguishable from endogenous fluorescence.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityCell-Permeant
ColorYellow-Green
Concentration5 mM
Detection MethodFluorescence
FormLiquid
Product LineELF™
Quantity1 mL
SubstratePhosphatase Substrate
Substrate PropertiesChemical Substrate
Target EnzymePhosphatases
Excitation/Emission345⁄530
For Use With (Equipment)Fluorescence Microscope, Microarray, Flow Cytometer
Label or DyeELF 97
Research CategoryDifferentiation
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

Can the ELF 97 reagent be applied to live cells?

ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can ELF 97 stained cell/tissue samples be multiplexed with antibody labeling?

No. The ELF 97 reagent does not covalently attach to any cellular components and may be washed away with any subsequent antibody labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.