Novex™ TBE Gels, 6%
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Invitrogen™

Novex™ TBE Gels, 6%

Novex™ TBE Gels 6% are designed to run on the XCell SureLock™ Mini-Cell and provide high-resolution analysis of restriction digests and PCR products.
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Catalog NumberDescriptionWells
EC62652BOXNovex TBE Gels, 6%, 12-well12-well
EC6261BOXNovex TBE Gels, 6%, 1-well1-well
EC6265BOXNovex TBE Gels, 6%, 10-well10-well
EC62655BOXNovex TBE Gels, 6%, 15-well15-well
Catalog number EC62652BOX
Price (USD)
239.00
Each
Add to cart
Description:
Novex TBE Gels, 6%, 12-well
Wells:
12-well
Request bulk or custom format
Price (USD)
239.00
Each
Add to cart
Novex™ TBE Gels 6% provide high-resolution analysis of restriction digests and PCR products. Designed to run on the XCell SureLock™ Mini-Cell, the polyacrylamide gels give sharp, clearly resolved, intense bands, and provide separations of double-strand DNA fragments from 65–250 bp.

Novex TBE Gels are available in a variety of well formats and gel percentages and can be stained by silver staining, ethidium­ bromide, and SYBR™ Green staining techniques after electrophoresis.

Advantages of TBE Gels for nucleic acid separation:
• High resolution and sensitivity with lower background staining
• Requires ∼10% sample concentration and volume of large or agarose gels
• Efficient blotting
• Easy extraction of DNA from gels
• Gel extraction does not interfere with enzymatic reactions
• Accurate and reproducible results

Formulation
Novex TBE gels are manufactured with high-purity Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended buffers
For optimum performance, Novex™ TBE Running Buffer and Novex™ Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex Hi-Density TBE Sample Buffer contains the tracking dyes Bromophenol Blue and Xylene Cyanol as well as the density agent Ficoll™, which yields sharper and straighter bands than conventional density agents.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionNovex TBE Gels, 6%, 12-well
For Use With (Equipment)XCell SureLock Mini-Cell
Gel Percentage6%
Gel TypeTBE
Product TypeTBE Gel
Separation Range65 to 250 bp
Wells12-well
Quantity10 Gels/Box
Sample TypeDouble-stranded DNA (dsDNA)
Shipping ConditionWet Ice
Unit SizeEach
Contents & Storage
• Catalog number refers to a single box of 10 gels

Store at 4°C.

Frequently asked questions (FAQs)

Can I stain my TBE gel? How?

Yes, you can stain your TBE gels with ethidium bromide, SYBR Green I, SYBR Green II, and the SilverXpress Silver Staining Kit. For ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light.

What is the difference in composition between the Invitrogen 6% TBE Gels and the Invitrogen 6% DNA Retardation Gels?

The Invitrogen 6% DNA Retardation Gels contain 0.5X TBE. Both gels will work for gel retardation; however, the 1X TBE in the Invitrogen 6% TBE Gels have a higher ionic environment, which may affect DNA-protein interactions. The 0.5X TBE used in the Invitrogen 6% DNA Retardation Gels usually works better, as it offers good fragment separation in electrophoresis yet has an ionic strength low enough to promote DNA-protein interactions.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What are the smallest fragments that can be visualized on the TBE gels?

On the Invitrogen 10% TBE gels, a 51 bp marker can be clearly seen. On the Invitrogen 20% TBE gel, the 18 and 12 bp markers can be clearly seen.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the concentration of EDTA in the TBE gels? How about glycerol?

The EDTA concentration in our TBE gels is 0.06% (w/v). The 20% TBE gels contain 4% glycerol for maximal resolution. All other TBE gels contain 0.8% glycerol in a layer that represents the bottom 9% of the gel. There is no glycerol in the rest of the gel.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I stain my TBE gel or my TBE-urea gel? How?

Yes, for ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

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